TY  - JOUR
  - Julio-Pieper, M,O'Mahony, CM,Clarke, G,Bravo, JA,Dinan, TG,Cryan, JF
  - 2012
  - January
  - Stress (Amsterdam, Netherlands)
  - Chronic stress-induced alterations in mouse colonic 5-HT and defecation responses are strain dependent
  - Validated
  - ()
  - Anxiety brain-gut axis depression diarrhoea irritable bowel syndrome serotonin IRRITABLE-BOWEL-SYNDROME CORTICOTROPIN-RELEASING-FACTOR TRYPTOPHAN-HYDROXYLASE GASTROINTESTINAL-TRACT SEROTONIN TRANSPORTER DEPRESSION ANXIETY MICE BEHAVIOR RECEPTOR
  - 15
  - 218
  - 226
  - Mood disorders and chronic stress are frequently associated with gastrointestinal (GI) symptoms including diarrhoea or constipation. Locally produced serotonin [5-hydroxytryptamine (5-HT)] regulates GI motility and is a key factor in the pathophysiology of stress-associated GI disorders. We aimed to establish whether chronic stress can differentially affect faecal output and colon 5-HT concentration in two inbred mouse strains: BALB/c and C57BL/6 which differ in their ability to cope with stress. Adult male BALB/c and C57BL/6 mice were restrained for 2 h daily for 10 days. Defecation was monitored during each stress session. Twenty-four hours after the last session of stress, plasma corticosterone concentration was higher than control in both strains, indicative of a physiological effect of chronic stress; however, stress-induced diarrhoea was more persistent in C57BL/6 mice. Basal concentration of colon 5-HT was higher in C57BL/6 mice, and stress elicited an increase in colon 5-HT only in this strain. Finally, naive BALB/c mice had a higher sensitivity (incidence of diarrhoea) to 5-HT (0.33 mg/kg, i.p.) than C57BL/6 mice. Our results suggest that differential defecation responses to stress may be associated with colon 5-HT concentration, which may in turn reflect the individual sensitivity to 5-HT. In addition, C57BL/6 mice emerge as a relevant model for studying GI alterations induced by chronic stress.
  - DOI 10.3109/10253890.2011.607524
DA  - 2012/01
ER  - 

@article{V160747712,
   = {Julio-Pieper,  M and O'Mahony,  CM and Clarke,  G and Bravo,  JA and Dinan,  TG and Cryan,  JF },
   = {2012},
   = {January},
   = {Stress (Amsterdam, Netherlands)},
   = {Chronic stress-induced alterations in mouse colonic 5-HT and defecation responses are strain dependent},
   = {Validated},
   = {()},
   = {Anxiety brain-gut axis depression diarrhoea irritable bowel syndrome serotonin IRRITABLE-BOWEL-SYNDROME CORTICOTROPIN-RELEASING-FACTOR TRYPTOPHAN-HYDROXYLASE GASTROINTESTINAL-TRACT SEROTONIN TRANSPORTER DEPRESSION ANXIETY MICE BEHAVIOR RECEPTOR},
   = {15},
  pages = {218--226},
   = {{Mood disorders and chronic stress are frequently associated with gastrointestinal (GI) symptoms including diarrhoea or constipation. Locally produced serotonin [5-hydroxytryptamine (5-HT)] regulates GI motility and is a key factor in the pathophysiology of stress-associated GI disorders. We aimed to establish whether chronic stress can differentially affect faecal output and colon 5-HT concentration in two inbred mouse strains: BALB/c and C57BL/6 which differ in their ability to cope with stress. Adult male BALB/c and C57BL/6 mice were restrained for 2 h daily for 10 days. Defecation was monitored during each stress session. Twenty-four hours after the last session of stress, plasma corticosterone concentration was higher than control in both strains, indicative of a physiological effect of chronic stress; however, stress-induced diarrhoea was more persistent in C57BL/6 mice. Basal concentration of colon 5-HT was higher in C57BL/6 mice, and stress elicited an increase in colon 5-HT only in this strain. Finally, naive BALB/c mice had a higher sensitivity (incidence of diarrhoea) to 5-HT (0.33 mg/kg, i.p.) than C57BL/6 mice. Our results suggest that differential defecation responses to stress may be associated with colon 5-HT concentration, which may in turn reflect the individual sensitivity to 5-HT. In addition, C57BL/6 mice emerge as a relevant model for studying GI alterations induced by chronic stress.}},
   = {DOI 10.3109/10253890.2011.607524},
  source = {IRIS}
}