IRIS publication 280546206
Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x
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TY - JOUR - Dudley, K.,Shanahan, F.,Burtenshaw, M.,Evans, E. P.,Ruddy, S.,Lyon, M. F. - 1991 - April - Genet Resgenet Res - Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x - Validated - () - 57 - 22 - 147 - 52 - The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1. - 0016-67230016-6723 DA - 1991/04 ER -
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@article{V280546206, = {Dudley, K. and Shanahan, F. and Burtenshaw, M. and Evans, E. P. and Ruddy, S. and Lyon, M. F. }, = {1991}, = {April}, = {Genet Resgenet Res}, = {Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x}, = {Validated}, = {()}, = {57}, = {22}, pages = {147--52}, = {{The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.}}, issn = {0016-67230016-6723}, source = {IRIS} }
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AUTHORS | Dudley, K.,Shanahan, F.,Burtenshaw, M.,Evans, E. P.,Ruddy, S.,Lyon, M. F. | ||
YEAR | 1991 | ||
MONTH | April | ||
JOURNAL_CODE | Genet Resgenet Res | ||
TITLE | Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 57 | ||
ISSUE | 22 | ||
START_PAGE | 147 | ||
END_PAGE | 52 | ||
ABSTRACT | The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1. | ||
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ISBN_ISSN | 0016-67230016-6723 | ||
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