TY  - JOUR
  - Dobson, A,Crispie, F,Rea, MC,O'Sullivan, O,Casey, PG,Lawlor, PG,Cotter, PD,Ross, P,Gardiner, GE,Hill, C
  - 2011
  - January
  - Fems Microbiology Ecology
  - Fate and efficacy of lacticin 3147-producing Lactococcus lactis in the mammalian gastrointestinal tract
  - Validated
  - ()
  - bacteriocin gastrointestinal tract lacticin 3147 colon model 2-COMPONENT LANTIBIOTIC LACTICIN-3147 ACID BACTERIA CAMPYLOBACTER-JEJUNI ANTIMICROBIAL ACTIVITY INTESTINAL MICROBIOTA ESCHERICHIA-COLI DIGESTIVE-TRACT CHEDDAR CHEESE SURVIVAL FOOD
  - 76
  - 602
  - 614
  - Gastrointestinal survival of the bacteriocin-producing strain, Lactococcus lactis DPC6520, was evaluated systematically in vitro and in vivo with a view to using this strain to deliver biologically active lacticin 3147, a broad-spectrum bacteriocin, to the gut. The activity of the lacticin 3147 producer was also evaluated against two clinically relevant pathogens: Clostridium difficile and Listeria monocytogenes. When suspended in an appropriate matrix, the lactococcal strain is capable of surviving simulated gastrointestinal juices similar to the porcine probiotic, Lactobacillus salivarius DPC6005. Upon administration of L. lactis DPC6520 to pigs (n=4), excretion rates of similar to 102-105 CFU g-1 faeces were observed by day 5. Although passage through the gastrointestinal tract (GIT) did not affect lacticin 3147 production by L. lactis DPC6520 isolates, activity was undetectable in faecal samples by an agar well diffusion assay. Furthermore, L. lactis DPC6520 had no inhibitory effect on C. difficile or other bacterial populations in a human distal colon model, while lactococcal counts declined 10 000-fold over 24 h. The lacticin 3147 producer failed to prevent L. monocytogenes infection in a mouse model, even though a mean L. lactis DPC6520 count of 4.7 x 104 CFU g-1 faeces was obtained over the 5-day administration period. These data demonstrate that L. lactis DPC6520 is capable of surviving transit through the GIT, and yet lacks antimicrobial efficacy in the models of infection used.
  - DOI 10.1111/j.1574-6941.2011.01069.x
DA  - 2011/01
ER  - 

@article{V160748799,
   = {Dobson,  A and Crispie,  F and Rea,  MC and O'Sullivan,  O and Casey,  PG and Lawlor,  PG and Cotter,  PD and Ross,  P and Gardiner,  GE and Hill,  C },
   = {2011},
   = {January},
   = {Fems Microbiology Ecology},
   = {Fate and efficacy of lacticin 3147-producing Lactococcus lactis in the mammalian gastrointestinal tract},
   = {Validated},
   = {()},
   = {bacteriocin gastrointestinal tract lacticin 3147 colon model 2-COMPONENT LANTIBIOTIC LACTICIN-3147 ACID BACTERIA CAMPYLOBACTER-JEJUNI ANTIMICROBIAL ACTIVITY INTESTINAL MICROBIOTA ESCHERICHIA-COLI DIGESTIVE-TRACT CHEDDAR CHEESE SURVIVAL FOOD},
   = {76},
  pages = {602--614},
   = {{Gastrointestinal survival of the bacteriocin-producing strain, Lactococcus lactis DPC6520, was evaluated systematically in vitro and in vivo with a view to using this strain to deliver biologically active lacticin 3147, a broad-spectrum bacteriocin, to the gut. The activity of the lacticin 3147 producer was also evaluated against two clinically relevant pathogens: Clostridium difficile and Listeria monocytogenes. When suspended in an appropriate matrix, the lactococcal strain is capable of surviving simulated gastrointestinal juices similar to the porcine probiotic, Lactobacillus salivarius DPC6005. Upon administration of L. lactis DPC6520 to pigs (n=4), excretion rates of similar to 102-105 CFU g-1 faeces were observed by day 5. Although passage through the gastrointestinal tract (GIT) did not affect lacticin 3147 production by L. lactis DPC6520 isolates, activity was undetectable in faecal samples by an agar well diffusion assay. Furthermore, L. lactis DPC6520 had no inhibitory effect on C. difficile or other bacterial populations in a human distal colon model, while lactococcal counts declined 10 000-fold over 24 h. The lacticin 3147 producer failed to prevent L. monocytogenes infection in a mouse model, even though a mean L. lactis DPC6520 count of 4.7 x 104 CFU g-1 faeces was obtained over the 5-day administration period. These data demonstrate that L. lactis DPC6520 is capable of surviving transit through the GIT, and yet lacks antimicrobial efficacy in the models of infection used.}},
   = {DOI 10.1111/j.1574-6941.2011.01069.x},
  source = {IRIS}
}