TY  - JOUR
  - Daly, KM,Upton, M,Sandiford, SK,Draper, LA,Wescombe, PA,Jack, RW,O'Connor, PM,Rossney, A,Gotz, F,Hill, C,Cotter, PD,Ross, RP,Tagg, JR
  - 2010
  - January
  - Journal of Bacteriology
  - Production of the Bsa Lantibiotic by Community-Acquired Staphylococcus aureus Strains
  - Validated
  - ()
  - PANTON-VALENTINE LEUKOCIDIN SOFT-TISSUE INFECTIONS METHICILLIN-RESISTANT STREPTOCOCCUS-MUTANS COMPARATIVE GENOMICS STRUCTURAL GENE PREPEPTIDE SEQUENCE BIOSYNTHESIS GENES BACILLUS-SUBTILIS DRUG-RESISTANCE
  - 192
  - 1131
  - 1142
  - Lantibiotics are antimicrobial peptides that have been the focus of much attention in recent years with a view to clinical, veterinary, and food applications. Although many lantibiotics are produced by food-grade bacteria or bacteria generally regarded as safe, some lantibiotics are produced by pathogens and, rather than contributing to food safety and/or health, add to the virulence potential of the producing strains. Indeed, genome sequencing has revealed the presence of genes apparently encoding a lantibiotic, designated Bsa (bacteriocin of Staphylococcus aureus), among clinical isolates of S. aureus and those associated with community-acquired methicillin-resistant S. aureus (MRSA) infections in particular. Here, we establish for the first time, through a combination of reverse genetics, mass spectrometry, and mutagenesis, that these genes encode a functional lantibiotic. We also reveal that Bsa is identical to the previously identified bacteriocin staphylococcin Au-26, produced by an S. aureus strain of vaginal origin. Our examination of MRSA isolates that produce the Panton-Valentine leukocidin demonstrates that many community-acquired S. aureus strains, and representatives of ST8 and ST80 in particular, are producers of Bsa. While possession of Bsa immunity genes does not significantly enhance resistance to the related lantibiotic gallidermin, the broad antimicrobial spectrum of Bsa strongly indicates that production of this bacteriocin confers a competitive ecological advantage on community-acquired S. aureus.
  - DOI 10.1128/JB.01375-09
DA  - 2010/01
ER  - 

@article{V160750600,
   = {Daly,  KM and Upton,  M and Sandiford,  SK and Draper,  LA and Wescombe,  PA and Jack,  RW and O'Connor,  PM and Rossney,  A and Gotz,  F and Hill,  C and Cotter,  PD and Ross,  RP and Tagg,  JR },
   = {2010},
   = {January},
   = {Journal of Bacteriology},
   = {Production of the Bsa Lantibiotic by Community-Acquired Staphylococcus aureus Strains},
   = {Validated},
   = {()},
   = {PANTON-VALENTINE LEUKOCIDIN SOFT-TISSUE INFECTIONS METHICILLIN-RESISTANT STREPTOCOCCUS-MUTANS COMPARATIVE GENOMICS STRUCTURAL GENE PREPEPTIDE SEQUENCE BIOSYNTHESIS GENES BACILLUS-SUBTILIS DRUG-RESISTANCE},
   = {192},
  pages = {1131--1142},
   = {{Lantibiotics are antimicrobial peptides that have been the focus of much attention in recent years with a view to clinical, veterinary, and food applications. Although many lantibiotics are produced by food-grade bacteria or bacteria generally regarded as safe, some lantibiotics are produced by pathogens and, rather than contributing to food safety and/or health, add to the virulence potential of the producing strains. Indeed, genome sequencing has revealed the presence of genes apparently encoding a lantibiotic, designated Bsa (bacteriocin of Staphylococcus aureus), among clinical isolates of S. aureus and those associated with community-acquired methicillin-resistant S. aureus (MRSA) infections in particular. Here, we establish for the first time, through a combination of reverse genetics, mass spectrometry, and mutagenesis, that these genes encode a functional lantibiotic. We also reveal that Bsa is identical to the previously identified bacteriocin staphylococcin Au-26, produced by an S. aureus strain of vaginal origin. Our examination of MRSA isolates that produce the Panton-Valentine leukocidin demonstrates that many community-acquired S. aureus strains, and representatives of ST8 and ST80 in particular, are producers of Bsa. While possession of Bsa immunity genes does not significantly enhance resistance to the related lantibiotic gallidermin, the broad antimicrobial spectrum of Bsa strongly indicates that production of this bacteriocin confers a competitive ecological advantage on community-acquired S. aureus.}},
   = {DOI 10.1128/JB.01375-09},
  source = {IRIS}
}