TY  - JOUR
  - Guinane, CM,Cotter, PD,Hill, C,Ross, RP
  - 2006
  - June
  - Fems Microbiology Letters
  - Spontaneous resistance in Lactococcus lactis IL1403 to the lantibiotic lacticin 3147
  - Validated
  - ()
  - Lactococcus lactis lacticin 3147 bacteriocin resistance LISTERIA-MONOCYTOGENES SCOTT CLASS-IIA BACTERIOCINS NISIN RESISTANCE FITNESS COSTS SENSITIVITY FOOD EXPRESSION INDUCTION TOLERANCE PATHOGENS
  - 260
  - 77
  - 83
  - The ability and frequency at which target organisms can develop resistance to bacteriocins is a crucial consideration in designing and implementing bacteriocin-based biocontrol strategies. Lactococcus lactis ssp. lactis IL1403 was used as a target strain in an attempt to determine the frequency at which spontaneously resistant mutants are likely to emerge to the lantibiotic lacticin 3147. Following a single exposure to lacticin 3147, resistant mutants only emerged at a low frequency (10(-8)-10(-9)) and were only able to withstand low levels of the bacteriocin (100 AU mL(-1)). However, exposure to increasing concentrations, in a stepwise manner, resulted in the isolation of eight mutants that were resistant to moderately higher levels of lacticin 3147 (up to 600 AU mL(-1)). Interestingly, in a number of cases cross-resistance to other lantibiotics such as nisin and lacticin 481 was observed, as was cross-resistance to environmental stresses such as salt. Finally, reduced adsorption of the bacteriocin in to the cell was documented for all resistant mutants.
  - DOI 10.1111/j.1574-6968.2006.00302.x
DA  - 2006/06
ER  - 

@article{V160753996,
   = {Guinane,  CM and Cotter,  PD and Hill,  C and Ross,  RP },
   = {2006},
   = {June},
   = {Fems Microbiology Letters},
   = {Spontaneous resistance in Lactococcus lactis IL1403 to the lantibiotic lacticin 3147},
   = {Validated},
   = {()},
   = {Lactococcus lactis lacticin 3147 bacteriocin resistance LISTERIA-MONOCYTOGENES SCOTT CLASS-IIA BACTERIOCINS NISIN RESISTANCE FITNESS COSTS SENSITIVITY FOOD EXPRESSION INDUCTION TOLERANCE PATHOGENS},
   = {260},
  pages = {77--83},
   = {{The ability and frequency at which target organisms can develop resistance to bacteriocins is a crucial consideration in designing and implementing bacteriocin-based biocontrol strategies. Lactococcus lactis ssp. lactis IL1403 was used as a target strain in an attempt to determine the frequency at which spontaneously resistant mutants are likely to emerge to the lantibiotic lacticin 3147. Following a single exposure to lacticin 3147, resistant mutants only emerged at a low frequency (10(-8)-10(-9)) and were only able to withstand low levels of the bacteriocin (100 AU mL(-1)). However, exposure to increasing concentrations, in a stepwise manner, resulted in the isolation of eight mutants that were resistant to moderately higher levels of lacticin 3147 (up to 600 AU mL(-1)). Interestingly, in a number of cases cross-resistance to other lantibiotics such as nisin and lacticin 481 was observed, as was cross-resistance to environmental stresses such as salt. Finally, reduced adsorption of the bacteriocin in to the cell was documented for all resistant mutants.}},
   = {DOI 10.1111/j.1574-6968.2006.00302.x},
  source = {IRIS}
}