TY  - JOUR
  - Sleator, RD,Wood, JM,Hill, C
  - 2003
  - March
  - Journal of Bacteriology
  - Transcriptional regulation and posttranslational activity of the betaine transporter BetL in Listeria monocytogenes are controlled by environmental salinity
  - Validated
  - ()
  - GENE-EXPRESSION SYSTEMS GRAM-POSITIVE BACTERIA GLYCINE-BETAINE LACTOCOCCUS-LACTIS MEMBRANE-VESICLES SALT TOLERANCE NISA PROMOTER GROWTH INDUCTION VIRULENCE
  - 185
  - 7140
  - 7144
  - While the genetic elements contributing to the salinity tolerance of Listeria monocytogenes have been well characterized, the regulatory signals and responses (genetic and/or biochemical) that govern these mechanisms have yet to be elucidated. Encoded by betL, the first genetic element to be linked to listerial osmotolerance, the secondary betaine uptake system BetL is a member of the betaine-carnitine-choline transporter family. Preceded by consensus sigma(A)- and sigma(B)-dependent promoter sites, betL is constitutively expressed and transcriptionally up-regulated in response to salt stress. The nisin-controlled expression system was used to achieve salinity-independent, controlled betL expression in Listeria. In the absence of NaCl-activated transcriptional control, BetL activity was found to be a function of environmental salinity, showing optimal activity in buffer supplemented with 1 to 2% NaCl (osmolality, 417 to 719 mosmol/kg). In addition, BetL was activated rapidly (half-life, 2 min) in response to an osmotic upshift imposed by adding 2% NaCl to 50 mM potassium phosphate buffer.
  - DOI 10.1128/JB.185.24.7140-7144.2003
DA  - 2003/03
ER  - 

@article{V160755416,
   = {Sleator,  RD and Wood,  JM and Hill,  C },
   = {2003},
   = {March},
   = {Journal of Bacteriology},
   = {Transcriptional regulation and posttranslational activity of the betaine transporter BetL in Listeria monocytogenes are controlled by environmental salinity},
   = {Validated},
   = {()},
   = {GENE-EXPRESSION SYSTEMS GRAM-POSITIVE BACTERIA GLYCINE-BETAINE LACTOCOCCUS-LACTIS MEMBRANE-VESICLES SALT TOLERANCE NISA PROMOTER GROWTH INDUCTION VIRULENCE},
   = {185},
  pages = {7140--7144},
   = {{While the genetic elements contributing to the salinity tolerance of Listeria monocytogenes have been well characterized, the regulatory signals and responses (genetic and/or biochemical) that govern these mechanisms have yet to be elucidated. Encoded by betL, the first genetic element to be linked to listerial osmotolerance, the secondary betaine uptake system BetL is a member of the betaine-carnitine-choline transporter family. Preceded by consensus sigma(A)- and sigma(B)-dependent promoter sites, betL is constitutively expressed and transcriptionally up-regulated in response to salt stress. The nisin-controlled expression system was used to achieve salinity-independent, controlled betL expression in Listeria. In the absence of NaCl-activated transcriptional control, BetL activity was found to be a function of environmental salinity, showing optimal activity in buffer supplemented with 1 to 2% NaCl (osmolality, 417 to 719 mosmol/kg). In addition, BetL was activated rapidly (half-life, 2 min) in response to an osmotic upshift imposed by adding 2% NaCl to 50 mM potassium phosphate buffer.}},
   = {DOI 10.1128/JB.185.24.7140-7144.2003},
  source = {IRIS}
}