TY  - JOUR
  - Hoffmann, RF,McLernon, S,Feeney, A,Hill, C,Sleator, RD
  - 2013
  - November
  - Bioengineered
  - A single point mutation in the listerial betL sigma(A)-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity
  - Validated
  - Altmetric: 2 ()
  - Listeria osmotolerance chill-tolerance salt stress twisted cells DEPENDENT TRANSPORT-SYSTEM ESCHERICHIA-COLI GLYCINE BETAINE BACILLUS-SUBTILIS SALT TOLERANCE GENE-EXPRESSION SALMONELLA-TYPHIMURIUM MONOCYTOGENES LO28 MURINE INFECTION BACTERIAL STRESS
  - 4
  - 401
  - 407
  - Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported "twisted" cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth.
  - 10.4161/bioe.24094
DA  - 2013/11
ER  - 

@article{V271354409,
   = {Hoffmann,  RF and McLernon,  S and Feeney,  A and Hill,  C and Sleator,  RD },
   = {2013},
   = {November},
   = {Bioengineered},
   = {A single point mutation in the listerial betL sigma(A)-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity},
   = {Validated},
   = {Altmetric: 2 ()},
   = {Listeria osmotolerance chill-tolerance salt stress twisted cells DEPENDENT TRANSPORT-SYSTEM ESCHERICHIA-COLI GLYCINE BETAINE BACILLUS-SUBTILIS SALT TOLERANCE GENE-EXPRESSION SALMONELLA-TYPHIMURIUM MONOCYTOGENES LO28 MURINE INFECTION BACTERIAL STRESS},
   = {4},
  pages = {401--407},
   = {{Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported "twisted" cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth.}},
   = {10.4161/bioe.24094},
  source = {IRIS}
}