TY  - JOUR
  - Culligan, EP,Sleator, RD,Marchesi, JR,Hill, C
  - 2014
  - July
  - Plos One
  - Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family beta-Carotene 15,15'-Monooxygenase
  - Validated
  - Altmetric: 7 ()
  - ESCHERICHIA-COLI LISTERIA-MONOCYTOGENES STRESS-RESPONSE GASTROINTESTINAL-TRACT STAPHYLOCOCCUS-AUREUS SEQUENCE ALIGNMENTS BACTERIAL STRESS OXIDATIVE STRESS BINDING-PROTEIN SIGMA-FACTOR
  - 9
  - The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family beta-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous beta-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.
  - 10.1371/journal.pone.0103318
DA  - 2014/07
ER  - 

@article{V279269362,
   = {Culligan,  EP and Sleator,  RD and Marchesi,  JR and Hill,  C },
   = {2014},
   = {July},
   = {Plos One},
   = {Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family beta-Carotene 15,15'-Monooxygenase},
   = {Validated},
   = {Altmetric: 7 ()},
   = {ESCHERICHIA-COLI LISTERIA-MONOCYTOGENES STRESS-RESPONSE GASTROINTESTINAL-TRACT STAPHYLOCOCCUS-AUREUS SEQUENCE ALIGNMENTS BACTERIAL STRESS OXIDATIVE STRESS BINDING-PROTEIN SIGMA-FACTOR},
   = {9},
   = {{The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family beta-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous beta-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.}},
   = {10.1371/journal.pone.0103318},
  source = {IRIS}
}