Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp globosum and its use for expression vector construction

Typeset version

 

TY  - JOUR
  - Sangrador-Vegas, A,Stanton, C,van Sinderen, D,Fitzgerald, GF,Ross, RP
  - 2007
  - September
  - Plasmid
  - Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp globosum and its use for expression vector construction
  - Validated
  - ()
  - Bifidobacterium Probiotic shuttle expression vector ROLLING-CIRCLE PLASMIDS NUCLEOTIDE-SEQUENCE MOLECULAR CHARACTERIZATION FUNCTIONAL-ANALYSIS GENOMIC DIVERSITY BACILLUS-SUBTILIS LONGUM REPLICATION GENE PROMOTERS
  - 58
  - 140
  - 147
  - Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter. (c) 2007 Elsevier Inc. All rights reserved.
  - 10.1016/j.plasmid.2007.02.004
DA  - 2007/09
ER  - 
@article{V243942571,
   = {Sangrador-Vegas,  A and Stanton,  C and van Sinderen,  D and Fitzgerald,  GF and Ross,  RP },
   = {2007},
   = {September},
   = {Plasmid},
   = {Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp globosum and its use for expression vector construction},
   = {Validated},
   = {()},
   = {Bifidobacterium Probiotic shuttle expression vector ROLLING-CIRCLE PLASMIDS NUCLEOTIDE-SEQUENCE MOLECULAR CHARACTERIZATION FUNCTIONAL-ANALYSIS GENOMIC DIVERSITY BACILLUS-SUBTILIS LONGUM REPLICATION GENE PROMOTERS},
   = {58},
  pages = {140--147},
   = {{Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter. (c) 2007 Elsevier Inc. All rights reserved.}},
   = {10.1016/j.plasmid.2007.02.004},
  source = {IRIS}
}
AUTHORSSangrador-Vegas, A,Stanton, C,van Sinderen, D,Fitzgerald, GF,Ross, RP
YEAR2007
MONTHSeptember
JOURNAL_CODEPlasmid
TITLECharacterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp globosum and its use for expression vector construction
STATUSValidated
TIMES_CITED()
SEARCH_KEYWORDBifidobacterium Probiotic shuttle expression vector ROLLING-CIRCLE PLASMIDS NUCLEOTIDE-SEQUENCE MOLECULAR CHARACTERIZATION FUNCTIONAL-ANALYSIS GENOMIC DIVERSITY BACILLUS-SUBTILIS LONGUM REPLICATION GENE PROMOTERS
VOLUME58
ISSUE
START_PAGE140
END_PAGE147
ABSTRACTBifidobacterium pseudolongum subsp. globosum DPC479 is an intestinally-derived strain which contains a plasmid, pASV479, 4.8 kb in size. This plasmid has a G + C content of 59% and contains six open reading frames (ORFs), four of which are cryptic. The other two ORFs have 47% and 54% identity, respectively, to the replication and FtsK-like proteins found in a Bifidobacterium breve NCFB 2258 plasmid, indicating that these plasmids, though isolated from differing Bifidobacterium species, are related. Using this plasmid as a backbone, an expression vector, pBIFRIBO, was constructed which exploits a bifidobacteria rRNA promoter. (c) 2007 Elsevier Inc. All rights reserved.
PUBLISHER_LOCATION
ISBN_ISSN
EDITION
URL
DOI_LINK10.1016/j.plasmid.2007.02.004
FUNDING_BODY
GRANT_DETAILS