In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species

Typeset version

 

TY  - JOUR
  - Tierney, J,Gowing, H,Van Sinderen, D,Flynn, S,Stanley, L,McHardy, N,Hallahan, S,Mulcahy, G
  - 2004
  - April
  - Veterinary Parasitology
  - In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species
  - Validated
  - ()
  - Eimeria tenella in vitro technique Lactobacillus sp. extracellular factors SALMONELLA-TYPHIMURIUM ACIDOPHILUS CELLS MICE IMMUNITY REUTERI FLORA LB
  - 122
  - 171
  - 182
  - The aim of this study was to determine the effects of indigenous chicken Lactobacillus species isolates from different parts of the gastrointestinal tract on Eimeria tenella invasion in vitro and to characterise the nature of inhibition, if any. The effects of competitive exclusion, steric interference and bacterial extracellular factors on E. tenella invasion were examined in an MDBK cell model. Several Lactobacillus species were initially isolated from chickens and identified by biochemical characteristics and 16S-rRNA. All Lactobacillus species isolates tested, significantly inhibited E. tenella invasion. Steric interference did not affect parasite invasion. Extracellular metabolic factors secreted by Lactobacillus species isolates into the surrounding media were shown to inhibit parasite invasion and these factors appeared to be heat stable. These results show that the natural microflora of poultry can provide a source of E. tenella-inhibiting Lactobacillus species in vitro, and thus may contribute to the control of Eimeria infection. (C) 2004 Elsevier B.V. All rights reserved.
  - DOI 10.1016/j.vetpar.2004.05.001
DA  - 2004/04
ER  - 
@article{V43337573,
   = {Tierney,  J and Gowing,  H and Van Sinderen,  D and Flynn,  S and Stanley,  L and McHardy,  N and Hallahan,  S and Mulcahy,  G },
   = {2004},
   = {April},
   = {Veterinary Parasitology},
   = {In vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species},
   = {Validated},
   = {()},
   = {Eimeria tenella in vitro technique Lactobacillus sp. extracellular factors SALMONELLA-TYPHIMURIUM ACIDOPHILUS CELLS MICE IMMUNITY REUTERI FLORA LB},
   = {122},
  pages = {171--182},
   = {{The aim of this study was to determine the effects of indigenous chicken Lactobacillus species isolates from different parts of the gastrointestinal tract on Eimeria tenella invasion in vitro and to characterise the nature of inhibition, if any. The effects of competitive exclusion, steric interference and bacterial extracellular factors on E. tenella invasion were examined in an MDBK cell model. Several Lactobacillus species were initially isolated from chickens and identified by biochemical characteristics and 16S-rRNA. All Lactobacillus species isolates tested, significantly inhibited E. tenella invasion. Steric interference did not affect parasite invasion. Extracellular metabolic factors secreted by Lactobacillus species isolates into the surrounding media were shown to inhibit parasite invasion and these factors appeared to be heat stable. These results show that the natural microflora of poultry can provide a source of E. tenella-inhibiting Lactobacillus species in vitro, and thus may contribute to the control of Eimeria infection. (C) 2004 Elsevier B.V. All rights reserved.}},
   = {DOI 10.1016/j.vetpar.2004.05.001},
  source = {IRIS}
}
AUTHORSTierney, J,Gowing, H,Van Sinderen, D,Flynn, S,Stanley, L,McHardy, N,Hallahan, S,Mulcahy, G
YEAR2004
MONTHApril
JOURNAL_CODEVeterinary Parasitology
TITLEIn vitro inhibition of Eimeria tenella invasion by indigenous chicken Lactobacillus species
STATUSValidated
TIMES_CITED()
SEARCH_KEYWORDEimeria tenella in vitro technique Lactobacillus sp. extracellular factors SALMONELLA-TYPHIMURIUM ACIDOPHILUS CELLS MICE IMMUNITY REUTERI FLORA LB
VOLUME122
ISSUE
START_PAGE171
END_PAGE182
ABSTRACTThe aim of this study was to determine the effects of indigenous chicken Lactobacillus species isolates from different parts of the gastrointestinal tract on Eimeria tenella invasion in vitro and to characterise the nature of inhibition, if any. The effects of competitive exclusion, steric interference and bacterial extracellular factors on E. tenella invasion were examined in an MDBK cell model. Several Lactobacillus species were initially isolated from chickens and identified by biochemical characteristics and 16S-rRNA. All Lactobacillus species isolates tested, significantly inhibited E. tenella invasion. Steric interference did not affect parasite invasion. Extracellular metabolic factors secreted by Lactobacillus species isolates into the surrounding media were shown to inhibit parasite invasion and these factors appeared to be heat stable. These results show that the natural microflora of poultry can provide a source of E. tenella-inhibiting Lactobacillus species in vitro, and thus may contribute to the control of Eimeria infection. (C) 2004 Elsevier B.V. All rights reserved.
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DOI_LINKDOI 10.1016/j.vetpar.2004.05.001
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