IRIS publication 725243
Molecular Dissection of A Bifidobacterial Replicon
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TY - JOUR - Cronin, M, Knobel, M, O'Connell-Motherway, M, Fitzgerald, GF, van Sinderen, D - 2007 - July - Applied and Environmental Microbiology - Molecular Dissection of A Bifidobacterial Replicon - Validated - () - 73 - 24 - 7858 - 7866 - The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA.. - DOI 10.1128/AEM.01630-07 DA - 2007/07 ER -
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@article{V725243, = {Cronin, M and Knobel, M and O'Connell-Motherway, M and Fitzgerald, GF and van Sinderen, D }, = {2007}, = {July}, = {Applied and Environmental Microbiology}, = {Molecular Dissection of A Bifidobacterial Replicon}, = {Validated}, = {()}, = {73}, = {24}, pages = {7858--7866}, = {{The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA..}}, = {DOI 10.1128/AEM.01630-07}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Cronin, M, Knobel, M, O'Connell-Motherway, M, Fitzgerald, GF, van Sinderen, D | ||
YEAR | 2007 | ||
MONTH | July | ||
JOURNAL_CODE | Applied and Environmental Microbiology | ||
TITLE | Molecular Dissection of A Bifidobacterial Replicon | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
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VOLUME | 73 | ||
ISSUE | 24 | ||
START_PAGE | 7858 | ||
END_PAGE | 7866 | ||
ABSTRACT | The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA.. | ||
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DOI_LINK | DOI 10.1128/AEM.01630-07 | ||
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