TY  - JOUR
  - Ventura, M,Lee, JH,Canchaya, C,Zink, R,Leahy, S,Moreno-Munoz, JA,O'Connell-Motherway, M,Higgins, D,Fitzgerald, GF,O'Sullivan, DJ,van Sinderen, D
  - 2005
  - December
  - Applied and Environmental Microbiology
  - Prophage-like elements in bifidobacteria: Insights from genomics, transcription, integration, distribution, and phylogenetic analysis
  - Validated
  - Altmetric: 2 ()
  - SITE-SPECIFIC RECOMBINATION STREPTOCOCCUS-THERMOPHILUS LACTOBACILLUS-JOHNSONII GENE-TRANSFER PHAGE SEQUENCE BACTERIA BACTERIOPHAGES IDENTIFICATION GENERATION
  - 71
  - 8692
  - 8705
  - So far, there is only fragmentary and unconfirmed information on bacteriophages infecting the genus Bifidobacterium. In this report we analyzed three prophage-like elements that are present in the genomes of Bifidobacterium breve UCC 2003, Bifidobacterium longum NCC 2705, and Bifidobacterium longum DJO10A, designated Bbr-1, Bl-1, and Blj-1, respectively. These prophagelike elements exhibit homology with genes of double-stranded DNA bacteriophages spanning a broad phylogenetic range of host bacteria and are surprisingly closely related to bacteriophages infecting low-G+C bacteria. All three prophage-like elements are integrated in a tRNA(Met) gene, which appears to be reconstructed following phage integration. Analysis of the distribution of this integration site in many bifidobacterial species revealed that the attB sites are well conserved. The Blj-1 prophage is 36.9 kb long and was induced when a B. longum DJO10A culture was exposed to mitomycin C or hydrogen peroxide. The Bbr-1 prophage-like element appears to consist of a noninducible 28.5-kb chimeric DNA fragment composed of a composite mobile element inserted into prophage-like sequences, which do not appear to be widely distributed among B. breve strains. Northern blot analysis of the Bbr-1 prophage-like element showed that large parts of its genome are transcriptionally silent. Interestingly, a gene predicted to encode an extracellular beta-glucosidase carried within the Bbr-1 prophage-like element was shown to be transcribed.
  - 10.1128/AEM.71.12.8692-8705.2005
DA  - 2005/12
ER  - 

@article{V243940723,
   = {Ventura,  M and Lee,  JH and Canchaya,  C and Zink,  R and Leahy,  S and Moreno-Munoz,  JA and O'Connell-Motherway,  M and Higgins,  D and Fitzgerald,  GF and O'Sullivan,  DJ and van Sinderen,  D },
   = {2005},
   = {December},
   = {Applied and Environmental Microbiology},
   = {Prophage-like elements in bifidobacteria: Insights from genomics, transcription, integration, distribution, and phylogenetic analysis},
   = {Validated},
   = {Altmetric: 2 ()},
   = {SITE-SPECIFIC RECOMBINATION STREPTOCOCCUS-THERMOPHILUS LACTOBACILLUS-JOHNSONII GENE-TRANSFER PHAGE SEQUENCE BACTERIA BACTERIOPHAGES IDENTIFICATION GENERATION},
   = {71},
  pages = {8692--8705},
   = {{So far, there is only fragmentary and unconfirmed information on bacteriophages infecting the genus Bifidobacterium. In this report we analyzed three prophage-like elements that are present in the genomes of Bifidobacterium breve UCC 2003, Bifidobacterium longum NCC 2705, and Bifidobacterium longum DJO10A, designated Bbr-1, Bl-1, and Blj-1, respectively. These prophagelike elements exhibit homology with genes of double-stranded DNA bacteriophages spanning a broad phylogenetic range of host bacteria and are surprisingly closely related to bacteriophages infecting low-G+C bacteria. All three prophage-like elements are integrated in a tRNA(Met) gene, which appears to be reconstructed following phage integration. Analysis of the distribution of this integration site in many bifidobacterial species revealed that the attB sites are well conserved. The Blj-1 prophage is 36.9 kb long and was induced when a B. longum DJO10A culture was exposed to mitomycin C or hydrogen peroxide. The Bbr-1 prophage-like element appears to consist of a noninducible 28.5-kb chimeric DNA fragment composed of a composite mobile element inserted into prophage-like sequences, which do not appear to be widely distributed among B. breve strains. Northern blot analysis of the Bbr-1 prophage-like element showed that large parts of its genome are transcriptionally silent. Interestingly, a gene predicted to encode an extracellular beta-glucosidase carried within the Bbr-1 prophage-like element was shown to be transcribed.}},
   = {10.1128/AEM.71.12.8692-8705.2005},
  source = {IRIS}
}