TY  - JOUR
  - Cronin, M,Knobel, M,O'Connell-Motherway, M,Fitzgerald, GF,van Sinderen, D
  - 2007
  - July
  - Applied and Environmental Microbiology
  - Molecular dissection of a Bifidobacterial replicon
  - Validated
  - ()
  - COMPLETE NUCLEOTIDE-SEQUENCE INITIATOR PROTEIN REP LACTIC-ACID BACTERIA CANCER GENE-THERAPY DELIVERY-SYSTEM CRYPTIC PLASMID VECTOR CONSTRUCTION ESCHERICHIA-COLI DNA-REPLICATION BREVE UCC2003
  - 73
  - 7858
  - 7866
  - The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA.
  - DOI 10.1128/AEM.01630-07
DA  - 2007/07
ER  - 

@article{V43335781,
   = {Cronin,  M and Knobel,  M and O'Connell-Motherway,  M and Fitzgerald,  GF and van Sinderen,  D },
   = {2007},
   = {July},
   = {Applied and Environmental Microbiology},
   = {Molecular dissection of a Bifidobacterial replicon},
   = {Validated},
   = {()},
   = {COMPLETE NUCLEOTIDE-SEQUENCE INITIATOR PROTEIN REP LACTIC-ACID BACTERIA CANCER GENE-THERAPY DELIVERY-SYSTEM CRYPTIC PLASMID VECTOR CONSTRUCTION ESCHERICHIA-COLI DNA-REPLICATION BREVE UCC2003},
   = {73},
  pages = {7858--7866},
   = {{The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA.}},
   = {DOI 10.1128/AEM.01630-07},
  source = {IRIS}
}