IRIS publication 43336473
Anatomy of a lactococcal phage tail
RIS format for Endnote and similar
TY - JOUR - Mc Grath, S,Neve, H,Seegers, JFML,Eijlander, R,Vegge, CS,Brondsted, L,Heller, KJ,Fitzgerald, GF,Vogensen, FK,van Sinderen, D - 2006 - June - Journal of Bacteriology - Anatomy of a lactococcal phage tail - Validated - () - BACTERIOPHAGE-LAMBDA TAIL LACTIC-ACID BACTERIA STRUCTURAL PROTEINS TEMPERATE BACTERIOPHAGE-TP901-1 MOLECULAR CHARACTERIZATION TRANSLATIONAL FRAMESHIFT COMPARATIVE GENOMICS DNA-SEQUENCE IDENTIFICATION GENES - 188 - 3972 - 3982 - Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae X has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with X with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with X and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures. - DOI 10.1128/JB.00024-06 DA - 2006/06 ER -
BIBTeX format for JabRef and similar
@article{V43336473, = {Mc Grath, S and Neve, H and Seegers, JFML and Eijlander, R and Vegge, CS and Brondsted, L and Heller, KJ and Fitzgerald, GF and Vogensen, FK and van Sinderen, D }, = {2006}, = {June}, = {Journal of Bacteriology}, = {Anatomy of a lactococcal phage tail}, = {Validated}, = {()}, = {BACTERIOPHAGE-LAMBDA TAIL LACTIC-ACID BACTERIA STRUCTURAL PROTEINS TEMPERATE BACTERIOPHAGE-TP901-1 MOLECULAR CHARACTERIZATION TRANSLATIONAL FRAMESHIFT COMPARATIVE GENOMICS DNA-SEQUENCE IDENTIFICATION GENES}, = {188}, pages = {3972--3982}, = {{Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae X has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with X with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with X and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures.}}, = {DOI 10.1128/JB.00024-06}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Mc Grath, S,Neve, H,Seegers, JFML,Eijlander, R,Vegge, CS,Brondsted, L,Heller, KJ,Fitzgerald, GF,Vogensen, FK,van Sinderen, D | ||
YEAR | 2006 | ||
MONTH | June | ||
JOURNAL_CODE | Journal of Bacteriology | ||
TITLE | Anatomy of a lactococcal phage tail | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | BACTERIOPHAGE-LAMBDA TAIL LACTIC-ACID BACTERIA STRUCTURAL PROTEINS TEMPERATE BACTERIOPHAGE-TP901-1 MOLECULAR CHARACTERIZATION TRANSLATIONAL FRAMESHIFT COMPARATIVE GENOMICS DNA-SEQUENCE IDENTIFICATION GENES | ||
VOLUME | 188 | ||
ISSUE | |||
START_PAGE | 3972 | ||
END_PAGE | 3982 | ||
ABSTRACT | Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae X has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with X with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with X and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures. | ||
PUBLISHER_LOCATION | |||
ISBN_ISSN | |||
EDITION | |||
URL | |||
DOI_LINK | DOI 10.1128/JB.00024-06 | ||
FUNDING_BODY | |||
GRANT_DETAILS |