TY  - JOUR
  - Sheehan, VM,Sleator, RD,Fitzgerald, GF,Hill, C
  - 2006
  - March
  - Applied and Environmental Microbiology
  - Heterologous expression of BetL, a betaine uptake system, enhances the stress tolerance of Lactobacillus salivarius UCC118
  - Validated
  - ()
  - LACTIC-ACID BACTERIA LISTERIA-MONOCYTOGENES LO28 GLYCINE BETAINE LACTOCOCCUS-LACTIS TRANSPORT-SYSTEM PROBIOTIC BACTERIA COMPATIBLE SOLUTES GENE-EXPRESSION SALT TOLERANCE NISA PROMOTER
  - 72
  - 2170
  - 2177
  - Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.
  - DOI 10.1128/AEM.72.3.2170-2177.2006
DA  - 2006/03
ER  - 

@article{V43336717,
   = {Sheehan,  VM and Sleator,  RD and Fitzgerald,  GF and Hill,  C },
   = {2006},
   = {March},
   = {Applied and Environmental Microbiology},
   = {Heterologous expression of BetL, a betaine uptake system, enhances the stress tolerance of Lactobacillus salivarius UCC118},
   = {Validated},
   = {()},
   = {LACTIC-ACID BACTERIA LISTERIA-MONOCYTOGENES LO28 GLYCINE BETAINE LACTOCOCCUS-LACTIS TRANSPORT-SYSTEM PROBIOTIC BACTERIA COMPATIBLE SOLUTES GENE-EXPRESSION SALT TOLERANCE NISA PROMOTER},
   = {72},
  pages = {2170--2177},
   = {{Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.}},
   = {DOI 10.1128/AEM.72.3.2170-2177.2006},
  source = {IRIS}
}