IRIS publication 43337524
The evaluation of a mupirocin-based selective medium for the enumeration of bifidobacteria from probiotic animal feed
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TY - JOUR - Simpson, PJ,Fitzgerald, GF,Stanton, C,Ross, RP - 2004 - April - Rna-A Publication of The Rna Society - The evaluation of a mupirocin-based selective medium for the enumeration of bifidobacteria from probiotic animal feed - Validated - () - bifidobacteria mupirocin probiotic animal feed FERMENTED DAIRY-PRODUCTS LACTOBACILLUS-ACIDOPHILUS ANTIMICROBIAL SUSCEPTIBILITY ENTEROCOCCUS-FAECIUM DISK DIFFUSION MILK-PRODUCTS AGAR CULTURES BIFIDUM - 57 - 9 - 16 - In this study, MRS medium supplemented with cysteine hydrochloride and mupirocin, termed Bifidobacterium selective medium (BSM) was found to be elective for bifidobacteria but inhibitory to a wide range of non-bifidobacteria strains commonly included in probiotic animal feed. Bacilli, lactobacilli, lactococci and streptococci failed to form colonies on BSM and enterococci, pediococci and propionibacteria formed colonies < 0.5 mm in diameter. Bifidobacteria formed colonies > 1 mm in size and could be readily distinguished. The addition of nystatin to BSM further inhibited Saccharomyces cerevisiae. BSM was successfully used to enumerate the bifidobacteria components, confirmed through fructose-6-phophate-phosphoketolase detection, present in two commercial probiotic feeds. The medium is recommended for the enumeration of bifidobacteria from animal feeds especially when not a numerically dominant component. (C) 2003 Elsevier B.V. All rights reserved. - DOI 10.1016/j.mimet.2003.11.010 DA - 2004/04 ER -
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@article{V43337524, = {Simpson, PJ and Fitzgerald, GF and Stanton, C and Ross, RP }, = {2004}, = {April}, = {Rna-A Publication of The Rna Society}, = {The evaluation of a mupirocin-based selective medium for the enumeration of bifidobacteria from probiotic animal feed}, = {Validated}, = {()}, = {bifidobacteria mupirocin probiotic animal feed FERMENTED DAIRY-PRODUCTS LACTOBACILLUS-ACIDOPHILUS ANTIMICROBIAL SUSCEPTIBILITY ENTEROCOCCUS-FAECIUM DISK DIFFUSION MILK-PRODUCTS AGAR CULTURES BIFIDUM}, = {57}, pages = {9--16}, = {{In this study, MRS medium supplemented with cysteine hydrochloride and mupirocin, termed Bifidobacterium selective medium (BSM) was found to be elective for bifidobacteria but inhibitory to a wide range of non-bifidobacteria strains commonly included in probiotic animal feed. Bacilli, lactobacilli, lactococci and streptococci failed to form colonies on BSM and enterococci, pediococci and propionibacteria formed colonies < 0.5 mm in diameter. Bifidobacteria formed colonies > 1 mm in size and could be readily distinguished. The addition of nystatin to BSM further inhibited Saccharomyces cerevisiae. BSM was successfully used to enumerate the bifidobacteria components, confirmed through fructose-6-phophate-phosphoketolase detection, present in two commercial probiotic feeds. The medium is recommended for the enumeration of bifidobacteria from animal feeds especially when not a numerically dominant component. (C) 2003 Elsevier B.V. All rights reserved.}}, = {DOI 10.1016/j.mimet.2003.11.010}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Simpson, PJ,Fitzgerald, GF,Stanton, C,Ross, RP | ||
YEAR | 2004 | ||
MONTH | April | ||
JOURNAL_CODE | Rna-A Publication of The Rna Society | ||
TITLE | The evaluation of a mupirocin-based selective medium for the enumeration of bifidobacteria from probiotic animal feed | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | bifidobacteria mupirocin probiotic animal feed FERMENTED DAIRY-PRODUCTS LACTOBACILLUS-ACIDOPHILUS ANTIMICROBIAL SUSCEPTIBILITY ENTEROCOCCUS-FAECIUM DISK DIFFUSION MILK-PRODUCTS AGAR CULTURES BIFIDUM | ||
VOLUME | 57 | ||
ISSUE | |||
START_PAGE | 9 | ||
END_PAGE | 16 | ||
ABSTRACT | In this study, MRS medium supplemented with cysteine hydrochloride and mupirocin, termed Bifidobacterium selective medium (BSM) was found to be elective for bifidobacteria but inhibitory to a wide range of non-bifidobacteria strains commonly included in probiotic animal feed. Bacilli, lactobacilli, lactococci and streptococci failed to form colonies on BSM and enterococci, pediococci and propionibacteria formed colonies < 0.5 mm in diameter. Bifidobacteria formed colonies > 1 mm in size and could be readily distinguished. The addition of nystatin to BSM further inhibited Saccharomyces cerevisiae. BSM was successfully used to enumerate the bifidobacteria components, confirmed through fructose-6-phophate-phosphoketolase detection, present in two commercial probiotic feeds. The medium is recommended for the enumeration of bifidobacteria from animal feeds especially when not a numerically dominant component. (C) 2003 Elsevier B.V. All rights reserved. | ||
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DOI_LINK | DOI 10.1016/j.mimet.2003.11.010 | ||
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