TY  - JOUR
  - Schirawski, J,Hagens, W,Fitzgerald, GF,van Sinderen, D
  - 2002
  - February
  - Applied and Environmental Microbiology
  - Molecular characterization of cadmium resistance in Streptococcus thermophilus strain 4134: An example of lateral gene transfer
  - Validated
  - ()
  - LACTIC-ACID BACTERIA HEAVY-METAL RESISTANCE AUREUS PLASMID PI258 STAPHYLOCOCCUS-AUREUS LACTOCOCCUS-LACTIS PHAGE-RESISTANCE LISTERIA-MONOCYTOGENES EXPRESSION CADA SEQUENCE
  - 68
  - 5508
  - 5516
  - Two genes (cadC(St) and cadA(St) [subscript St represents Streptococcus thermophilus]), located on the chromosome of S. thermophilus 4134, were shown to constitute a cadmium/zinc resistance cassette. The genes seem to be organized in an operon, and their transcription is cadmium dependent in vivo. The proposed product of the cadA open reading frame (CadA(St)) is highly similar to P-type cadmium efflux ATPases, whereas the predicted protein encoded by cadC, (CadC(St)) shows high similarity to ArsR-type regulatory proteins. The observed homologies and G+C content of this cassette and surrounding regions suggest that this DNA was derived from Lactococcus lactis and may have been introduced relatively recently into the S. thermophilus 4134 genome by a lateral gene transfer event. The complete cassette confers cadmium and zinc resistance to both S. thermophilus and L. lactis, but expression of cadA(St) alone is sufficient to give resistance. By using electrophoretic mobility shift assays it was shown that the CadC(St) protein is a DNA binding protein that binds specifically to its own promoter region, possibly to two copies of an inverted repeat, and that this CadC(St)-DNA interaction is lost in the presence of cadmium. Using lacZ fusion constructs it was shown that the cadmium-dependent expression of CadA(St) is mediated by the negative regulator CadC(St). A model for the regulation of the expression of cadmium resistance in S. thermophilus is discussed.
  - DOI 10.1128/AEM.68.11.5508-5516.2002
DA  - 2002/02
ER  - 

@article{V43338173,
   = {Schirawski,  J and Hagens,  W and Fitzgerald,  GF and van Sinderen,  D },
   = {2002},
   = {February},
   = {Applied and Environmental Microbiology},
   = {Molecular characterization of cadmium resistance in Streptococcus thermophilus strain 4134: An example of lateral gene transfer},
   = {Validated},
   = {()},
   = {LACTIC-ACID BACTERIA HEAVY-METAL RESISTANCE AUREUS PLASMID PI258 STAPHYLOCOCCUS-AUREUS LACTOCOCCUS-LACTIS PHAGE-RESISTANCE LISTERIA-MONOCYTOGENES EXPRESSION CADA SEQUENCE},
   = {68},
  pages = {5508--5516},
   = {{Two genes (cadC(St) and cadA(St) [subscript St represents Streptococcus thermophilus]), located on the chromosome of S. thermophilus 4134, were shown to constitute a cadmium/zinc resistance cassette. The genes seem to be organized in an operon, and their transcription is cadmium dependent in vivo. The proposed product of the cadA open reading frame (CadA(St)) is highly similar to P-type cadmium efflux ATPases, whereas the predicted protein encoded by cadC, (CadC(St)) shows high similarity to ArsR-type regulatory proteins. The observed homologies and G+C content of this cassette and surrounding regions suggest that this DNA was derived from Lactococcus lactis and may have been introduced relatively recently into the S. thermophilus 4134 genome by a lateral gene transfer event. The complete cassette confers cadmium and zinc resistance to both S. thermophilus and L. lactis, but expression of cadA(St) alone is sufficient to give resistance. By using electrophoretic mobility shift assays it was shown that the CadC(St) protein is a DNA binding protein that binds specifically to its own promoter region, possibly to two copies of an inverted repeat, and that this CadC(St)-DNA interaction is lost in the presence of cadmium. Using lacZ fusion constructs it was shown that the cadmium-dependent expression of CadA(St) is mediated by the negative regulator CadC(St). A model for the regulation of the expression of cadmium resistance in S. thermophilus is discussed.}},
   = {DOI 10.1128/AEM.68.11.5508-5516.2002},
  source = {IRIS}
}