TY  - JOUR
  - Labarre, C,Schirawski, J,van der Zwet, A,Fitzgerald, GF,van Sinderen, D
  - 2001
  - January
  - Fems Microbiology Letters
  - Insertional mutagenesis of an industrial strain of Streptococcus thermophilus
  - Validated
  - ()
  - mutagenesis industrial microorganism oligopeptide transport Streptococcus thermophilus OLIGOPEPTIDE TRANSPORT PLASMID SYSTEM GENES
  - 200
  - 85
  - 90
  - Random mutagenesis of an industrial strain of Streptococcus thermophilus was achieved through an adapted version of a two-plasmid system. The mutagenesis strategy is based on random integration of derivatives of the non-replicative (Rep(-)) plasmid pORI19 by means of homologous recombination following a temperature shift that eliminates replication of the temperature-sensitive (Rep(ts)) helper plasmid pVE6007. In this way mutants were generated which were affected in bacteriophage sensitivity or sucrose metabolism. Homologues were identified of a protein related to folate metabolism from a bacteriophage-resistant mutant and of two subunits of an oligopeptide transport system from a mutant deficient in sucrose utilisation. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
DA  - 2001/01
ER  - 

@article{V43338604,
   = {Labarre,  C and Schirawski,  J and van der Zwet,  A and Fitzgerald,  GF and van Sinderen,  D },
   = {2001},
   = {January},
   = {Fems Microbiology Letters},
   = {Insertional mutagenesis of an industrial strain of Streptococcus thermophilus},
   = {Validated},
   = {()},
   = {mutagenesis industrial microorganism oligopeptide transport Streptococcus thermophilus OLIGOPEPTIDE TRANSPORT PLASMID SYSTEM GENES},
   = {200},
  pages = {85--90},
   = {{Random mutagenesis of an industrial strain of Streptococcus thermophilus was achieved through an adapted version of a two-plasmid system. The mutagenesis strategy is based on random integration of derivatives of the non-replicative (Rep(-)) plasmid pORI19 by means of homologous recombination following a temperature shift that eliminates replication of the temperature-sensitive (Rep(ts)) helper plasmid pVE6007. In this way mutants were generated which were affected in bacteriophage sensitivity or sucrose metabolism. Homologues were identified of a protein related to folate metabolism from a bacteriophage-resistant mutant and of two subunits of an oligopeptide transport system from a mutant deficient in sucrose utilisation. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.}},
  source = {IRIS}
}