IRIS publication 43340075
The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanism, one which affects phage DNA penetration
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TY - JOUR - Garvey, P,Hill, C,Fitzgerald, GF - 1996 - February - Applied and Environmental Microbiology - The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanism, one which affects phage DNA penetration - Validated - () - STREPTOCOCCUS-LACTIS CONJUGAL TRANSFER RESTRICTION CREMORIS PTR2030 INFECTION PROPHAGE SYSTEMS PROTEIN NISIN - 62 - 676 - 679 - The lactococcal plasmid pNP40 mediates insensitivity to phi c2 by an early-acting phage resistance mechanism in addition to the previously identified abortive infection system, AbiF, in the Lactococcus lactis subsp, lactis MG1614 background. A second abortive infection determinant on pNP40, AbiE, does not confer resistance to phi c2. The early-acting mechanism on pNP40 does not prevent phage adsorption nor does it appear to operate by restriction/modification. Phage DNA was not detected in pNP40-containing cells until 30 min following exposure to phi c2 compared with 5 min in a sensitive host; however, electroporation of phage DNA into resistant hosts resulted in the release of phage progeny from a dramatically elevated number of cells compared with conventionally infected hosts. It appears therefore that pNP40 encodes a novel phage resistance mechanism which blocks DNA penetration specifically for phi c2. DA - 1996/02 ER -
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@article{V43340075, = {Garvey, P and Hill, C and Fitzgerald, GF }, = {1996}, = {February}, = {Applied and Environmental Microbiology}, = {The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanism, one which affects phage DNA penetration}, = {Validated}, = {()}, = {STREPTOCOCCUS-LACTIS CONJUGAL TRANSFER RESTRICTION CREMORIS PTR2030 INFECTION PROPHAGE SYSTEMS PROTEIN NISIN}, = {62}, pages = {676--679}, = {{The lactococcal plasmid pNP40 mediates insensitivity to phi c2 by an early-acting phage resistance mechanism in addition to the previously identified abortive infection system, AbiF, in the Lactococcus lactis subsp, lactis MG1614 background. A second abortive infection determinant on pNP40, AbiE, does not confer resistance to phi c2. The early-acting mechanism on pNP40 does not prevent phage adsorption nor does it appear to operate by restriction/modification. Phage DNA was not detected in pNP40-containing cells until 30 min following exposure to phi c2 compared with 5 min in a sensitive host; however, electroporation of phage DNA into resistant hosts resulted in the release of phage progeny from a dramatically elevated number of cells compared with conventionally infected hosts. It appears therefore that pNP40 encodes a novel phage resistance mechanism which blocks DNA penetration specifically for phi c2.}}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Garvey, P,Hill, C,Fitzgerald, GF | ||
YEAR | 1996 | ||
MONTH | February | ||
JOURNAL_CODE | Applied and Environmental Microbiology | ||
TITLE | The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanism, one which affects phage DNA penetration | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | STREPTOCOCCUS-LACTIS CONJUGAL TRANSFER RESTRICTION CREMORIS PTR2030 INFECTION PROPHAGE SYSTEMS PROTEIN NISIN | ||
VOLUME | 62 | ||
ISSUE | |||
START_PAGE | 676 | ||
END_PAGE | 679 | ||
ABSTRACT | The lactococcal plasmid pNP40 mediates insensitivity to phi c2 by an early-acting phage resistance mechanism in addition to the previously identified abortive infection system, AbiF, in the Lactococcus lactis subsp, lactis MG1614 background. A second abortive infection determinant on pNP40, AbiE, does not confer resistance to phi c2. The early-acting mechanism on pNP40 does not prevent phage adsorption nor does it appear to operate by restriction/modification. Phage DNA was not detected in pNP40-containing cells until 30 min following exposure to phi c2 compared with 5 min in a sensitive host; however, electroporation of phage DNA into resistant hosts resulted in the release of phage progeny from a dramatically elevated number of cells compared with conventionally infected hosts. It appears therefore that pNP40 encodes a novel phage resistance mechanism which blocks DNA penetration specifically for phi c2. | ||
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