TY  - JOUR
  - LUCEY, M,DALY, C,FITZGERALD, GF
  - 1992
  - October
  - Journal of General Microbiology
  - CELL-SURFACE CHARACTERISTICS OF LACTOCOCCUS-LACTIS HARBORING PCI528, A 46 KB PLASMID ENCODING INHIBITION OF BACTERIOPHAGE ADSORPTION
  - Validated
  - ()
  - SUBSP CREMORIS SK110 PHAGE-INSENSITIVE STRAIN STREPTOCOCCUS-LACTIS ABORTIVE INFECTION ACID BACTERIA POLYSACCHARIDE RESTRICTION RESISTANCE MECHANISMS DEFENSE
  - 138
  - 2137
  - 2143
  - pCI528 is a 46 kb plasmid, originally isolated from Lactococcus lactis subsp. cremoris UC503, which causes a decrease in bacteriophage adsorption in lactococcal strains. Cell surface characteristics of two pCI528-containing strains, L. lactis subsp. cremoris UC503 and L. lactis subsp. lactis UC505, were compared with those of their isogenic pCI528-cured derivatives, UC563 and MG1363, respectively. Following centrifugation, strains containing pCI528 produced a loose fluffy pellet. The presence of pCI528 also caused a 'hard' colony morphology on agar media. The fluffy pellet effect mediated by pCI528 could be eliminated by washing cells in 0.05 M-NaOH, which also restored full sensitivity to phage in the L. lactis subsp. lactis UC505 background. GLC analysis of cell wall material indicated that pCI528-containing hosts had significantly elevated levels of both galactose and rhamnose. Marked alterations in the cell surface of strains harbouring pCI528 were observed in electron micrographs.
DA  - 1992/10
ER  - 

@article{V43340313,
   = {LUCEY,  M and DALY,  C and FITZGERALD,  GF },
   = {1992},
   = {October},
   = {Journal of General Microbiology},
   = {CELL-SURFACE CHARACTERISTICS OF LACTOCOCCUS-LACTIS HARBORING PCI528, A 46 KB PLASMID ENCODING INHIBITION OF BACTERIOPHAGE ADSORPTION},
   = {Validated},
   = {()},
   = {SUBSP CREMORIS SK110 PHAGE-INSENSITIVE STRAIN STREPTOCOCCUS-LACTIS ABORTIVE INFECTION ACID BACTERIA POLYSACCHARIDE RESTRICTION RESISTANCE MECHANISMS DEFENSE},
   = {138},
  pages = {2137--2143},
   = {{pCI528 is a 46 kb plasmid, originally isolated from Lactococcus lactis subsp. cremoris UC503, which causes a decrease in bacteriophage adsorption in lactococcal strains. Cell surface characteristics of two pCI528-containing strains, L. lactis subsp. cremoris UC503 and L. lactis subsp. lactis UC505, were compared with those of their isogenic pCI528-cured derivatives, UC563 and MG1363, respectively. Following centrifugation, strains containing pCI528 produced a loose fluffy pellet. The presence of pCI528 also caused a 'hard' colony morphology on agar media. The fluffy pellet effect mediated by pCI528 could be eliminated by washing cells in 0.05 M-NaOH, which also restored full sensitivity to phage in the L. lactis subsp. lactis UC505 background. GLC analysis of cell wall material indicated that pCI528-containing hosts had significantly elevated levels of both galactose and rhamnose. Marked alterations in the cell surface of strains harbouring pCI528 were observed in electron micrographs.}},
  source = {IRIS}
}