IRIS publication 43340429
SCRFI RESTRICTION-MODIFICATION SYSTEM OF LACTOCOCCUS-LACTIS SUBSP CREMORIS UC503 - CLONING AND CHARACTERIZATION OF 2 SCRFI METHYLASE GENES
RIS format for Endnote and similar
TY - JOUR - DAVIS, R,VANDERLELIE, D,MERCENIER, A,DALY, C,FITZGERALD, GF - 1993 - March - Applied and Environmental Microbiology - SCRFI RESTRICTION-MODIFICATION SYSTEM OF LACTOCOCCUS-LACTIS SUBSP CREMORIS UC503 - CLONING AND CHARACTERIZATION OF 2 SCRFI METHYLASE GENES - Validated - () - ESCHERICHIA-COLI K-12 STREPTOCOCCUS-LACTIS II RESTRICTION DNA PLASMID BACTERIOPHAGE METHYLTRANSFERASE RECOMBINANTS EXPRESSION MECHANISMS - 59 - 777 - 785 - Two genes from the total genomic DNA of dairy starter culture Lactococcus lactis subsp. cremoris UC503, encoding ScrFI modification enzymes, have been cloned and expressed in Escherichia coli. No homology between the two methylase genes was detected, and inverse polymerase chain reaction of flanking chromosomal DNA indicated that both were linked on the Lactococcus genome. Neither clone encoded the cognate endonuclease. The DNA sequence of one of the methylase genes (encoded by pCI931M) was determined and consisted of an open reading frame 1,170 bp long, which could encode a protein of 389 amino acids (M(r)., 44.5). The amino acid sequence contained the highly characteristic motifs of an m5C methylase. Extensive regions of homology were observed with the methylases of NlaX, EcoRII, and Dcm. DA - 1993/03 ER -
BIBTeX format for JabRef and similar
@article{V43340429, = {DAVIS, R and VANDERLELIE, D and MERCENIER, A and DALY, C and FITZGERALD, GF }, = {1993}, = {March}, = {Applied and Environmental Microbiology}, = {SCRFI RESTRICTION-MODIFICATION SYSTEM OF LACTOCOCCUS-LACTIS SUBSP CREMORIS UC503 - CLONING AND CHARACTERIZATION OF 2 SCRFI METHYLASE GENES}, = {Validated}, = {()}, = {ESCHERICHIA-COLI K-12 STREPTOCOCCUS-LACTIS II RESTRICTION DNA PLASMID BACTERIOPHAGE METHYLTRANSFERASE RECOMBINANTS EXPRESSION MECHANISMS}, = {59}, pages = {777--785}, = {{Two genes from the total genomic DNA of dairy starter culture Lactococcus lactis subsp. cremoris UC503, encoding ScrFI modification enzymes, have been cloned and expressed in Escherichia coli. No homology between the two methylase genes was detected, and inverse polymerase chain reaction of flanking chromosomal DNA indicated that both were linked on the Lactococcus genome. Neither clone encoded the cognate endonuclease. The DNA sequence of one of the methylase genes (encoded by pCI931M) was determined and consisted of an open reading frame 1,170 bp long, which could encode a protein of 389 amino acids (M(r)., 44.5). The amino acid sequence contained the highly characteristic motifs of an m5C methylase. Extensive regions of homology were observed with the methylases of NlaX, EcoRII, and Dcm.}}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | DAVIS, R,VANDERLELIE, D,MERCENIER, A,DALY, C,FITZGERALD, GF | ||
YEAR | 1993 | ||
MONTH | March | ||
JOURNAL_CODE | Applied and Environmental Microbiology | ||
TITLE | SCRFI RESTRICTION-MODIFICATION SYSTEM OF LACTOCOCCUS-LACTIS SUBSP CREMORIS UC503 - CLONING AND CHARACTERIZATION OF 2 SCRFI METHYLASE GENES | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | ESCHERICHIA-COLI K-12 STREPTOCOCCUS-LACTIS II RESTRICTION DNA PLASMID BACTERIOPHAGE METHYLTRANSFERASE RECOMBINANTS EXPRESSION MECHANISMS | ||
VOLUME | 59 | ||
ISSUE | |||
START_PAGE | 777 | ||
END_PAGE | 785 | ||
ABSTRACT | Two genes from the total genomic DNA of dairy starter culture Lactococcus lactis subsp. cremoris UC503, encoding ScrFI modification enzymes, have been cloned and expressed in Escherichia coli. No homology between the two methylase genes was detected, and inverse polymerase chain reaction of flanking chromosomal DNA indicated that both were linked on the Lactococcus genome. Neither clone encoded the cognate endonuclease. The DNA sequence of one of the methylase genes (encoded by pCI931M) was determined and consisted of an open reading frame 1,170 bp long, which could encode a protein of 389 amino acids (M(r)., 44.5). The amino acid sequence contained the highly characteristic motifs of an m5C methylase. Extensive regions of homology were observed with the methylases of NlaX, EcoRII, and Dcm. | ||
PUBLISHER_LOCATION | |||
ISBN_ISSN | |||
EDITION | |||
URL | |||
DOI_LINK | |||
FUNDING_BODY | |||
GRANT_DETAILS |