TY  - JOUR
  - HILL, C,DALY, C,FITZGERALD, GF
  - 1991
  - June
  - Fems Microbiology Letters
  - ISOLATION OF CHROMOSOMAL MUTATIONS OF LACTOCOCCUS-LACTIS SUBSP LACTIS BIOVAR DIACETYLACTIS 18-16 AFTER INTRODUCTION OF TN919
  - Validated
  - ()
  - LACTOCOCCUS TN919 TRANSPOSON MUTAGENESIS CITRITASE MALTOSE STREPTOCOCCUS-LACTIS TRANSPOSON TN919 ESCHERICHIA-COLI PLASMID DNA BACTERIA CLONING
  - 81
  - 135
  - 140
  - The conjugative transposon Tn919 was introduced at high frequency to L. lactis subsp. lactis biovar. diacetylactis 18-16 and transconjugants were screened for mutations in two chromosomally located genotypes; citrate metabolism and maltose utilization. A citrate negative mutant, lacking citritase activity, was isolated at a frequency of 1.18 x 10(-4). The mutant, 18-16C5, contained a single copy of Tn919 in a chromosomal location. A junction fragment of Tn919::18-16C5 chromosomal DNA was cloned in Escherichia coli. Mutations in maltose metabolism were detected at a frequency of 4.0 x 10(-4). No mutants were detected when Tn919 was not introduced. Reversion to a Mal+ phenotype occurred at high frequency, but was not due to Tn919 transposition.
DA  - 1991/06
ER  - 

@article{V43340737,
   = {HILL,  C and DALY,  C and FITZGERALD,  GF },
   = {1991},
   = {June},
   = {Fems Microbiology Letters},
   = {ISOLATION OF CHROMOSOMAL MUTATIONS OF LACTOCOCCUS-LACTIS SUBSP LACTIS BIOVAR DIACETYLACTIS 18-16 AFTER INTRODUCTION OF TN919},
   = {Validated},
   = {()},
   = {LACTOCOCCUS TN919 TRANSPOSON MUTAGENESIS CITRITASE MALTOSE STREPTOCOCCUS-LACTIS TRANSPOSON TN919 ESCHERICHIA-COLI PLASMID DNA BACTERIA CLONING},
   = {81},
  pages = {135--140},
   = {{The conjugative transposon Tn919 was introduced at high frequency to L. lactis subsp. lactis biovar. diacetylactis 18-16 and transconjugants were screened for mutations in two chromosomally located genotypes; citrate metabolism and maltose utilization. A citrate negative mutant, lacking citritase activity, was isolated at a frequency of 1.18 x 10(-4). The mutant, 18-16C5, contained a single copy of Tn919 in a chromosomal location. A junction fragment of Tn919::18-16C5 chromosomal DNA was cloned in Escherichia coli. Mutations in maltose metabolism were detected at a frequency of 4.0 x 10(-4). No mutants were detected when Tn919 was not introduced. Reversion to a Mal+ phenotype occurred at high frequency, but was not due to Tn919 transposition.}},
  source = {IRIS}
}