TY  - JOUR
  - COFFEY, AG,FITZGERALD, GF,DALY, C
  - 1991
  - June
  - Journal of General Microbiology
  - CLONING AND CHARACTERIZATION OF THE DETERMINANT FOR ABORTIVE INFECTION OF BACTERIOPHAGE FROM LACTOCOCCAL PLASMID PCI829
  - Validated
  - ()
  - ENCODING PHAGE RESISTANCE LACTIC STREPTOCOCCI CONJUGAL TRANSFER FERMENTING ABILITY DNA FRAGMENTS LACTOSE IDENTIFICATION MECHANISMS SENSITIVITY PTR2030
  - 137
  - 1355
  - 1362
  - The genetic determinant for abortive infection of bacteriophage (Abi) from the lactococcal plasmid pC1829 was cloned on a 6.2 kb StuI fragment in Escherichia coli using the shuttle vector pSA3. In Lactococcus lactis subsp. lactis MG1363Sm the resulting recombinant plasmid pC1816 conferred complete insensitivity to the small isometric-headed phage 712 and a reduced plaque size in the case of the prolate-headed phage c2. The determinant was further localized by subcloning and nuclease Bal31 deletion analysis; approximately 2.0 kb of DNA was essential for the expression of the Abi+ phenotype. Nucleotide sequence analysis of this region revealed a putative open reading frame of 1887 base pairs preceded by a putative promotor sequence and ribosome-binding site which exhibited similarity to consensus E. coli and Bacillus subtilis transcription/translation signals. Hybridization experiments indicated that this region was not homologous to the abi determinant from the phenotypically similar lactococcal plasmid pC1750.
DA  - 1991/06
ER  - 

@article{V43340810,
   = {COFFEY,  AG and FITZGERALD,  GF and DALY,  C },
   = {1991},
   = {June},
   = {Journal of General Microbiology},
   = {CLONING AND CHARACTERIZATION OF THE DETERMINANT FOR ABORTIVE INFECTION OF BACTERIOPHAGE FROM LACTOCOCCAL PLASMID PCI829},
   = {Validated},
   = {()},
   = {ENCODING PHAGE RESISTANCE LACTIC STREPTOCOCCI CONJUGAL TRANSFER FERMENTING ABILITY DNA FRAGMENTS LACTOSE IDENTIFICATION MECHANISMS SENSITIVITY PTR2030},
   = {137},
  pages = {1355--1362},
   = {{The genetic determinant for abortive infection of bacteriophage (Abi) from the lactococcal plasmid pC1829 was cloned on a 6.2 kb StuI fragment in Escherichia coli using the shuttle vector pSA3. In Lactococcus lactis subsp. lactis MG1363Sm the resulting recombinant plasmid pC1816 conferred complete insensitivity to the small isometric-headed phage 712 and a reduced plaque size in the case of the prolate-headed phage c2. The determinant was further localized by subcloning and nuclease Bal31 deletion analysis; approximately 2.0 kb of DNA was essential for the expression of the Abi+ phenotype. Nucleotide sequence analysis of this region revealed a putative open reading frame of 1887 base pairs preceded by a putative promotor sequence and ribosome-binding site which exhibited similarity to consensus E. coli and Bacillus subtilis transcription/translation signals. Hybridization experiments indicated that this region was not homologous to the abi determinant from the phenotypically similar lactococcal plasmid pC1750.}},
  source = {IRIS}
}