Identification of a DNA region from lactococcal phage sk1 protecting phage 712 from the abortive infection mechanism AbiF

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TY  - JOUR
  - Rince, A,Tangney, M,Fitzgerald, GF
  - 2000
  - January
  - Fems Microbiology Letters
  - Identification of a DNA region from lactococcal phage sk1 protecting phage 712 from the abortive infection mechanism AbiF
  - Validated
  - ()
  - small isometric-headed phage 712 AbiF abortive infection mechanism PLASMID PNP40 LACTIS BACTERIOPHAGE-SK1 RESISTANCE TRANSCRIPTION STREPTOCOCCI SEQUENCE CLONING OPERON
  - 182
  - 185
  - 191
  - Bacteriophage 712 is a small isometric-headed phage which is sensitive to the lactococcal abortive infection mechanism AbiF. Its 29.6-kb DNA genome was characterized by restriction mapping and transcriptional analysis. Construction of a gene bank of lactococcal phage ski, which is insensitive to the action of AbiF, in Lactococcus lactis containing AbiF resulted in the identification of a 324-bp DNA fragment which reduced the effect of the abortive infection mechanism on phage 712. Analysis of this region provided evidence that the action of AbiF is related to the cos ends of small isometric-headed phages. Sequence analysis of a 3.2-kb segment containing the middle operon and the cos ends of phage 712 genome allowed comparison of this part of the phage 712 genome with the equivalent sequences of four other small isometric-headed phages. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
DA  - 2000/01
ER  - 
@article{V70046843,
   = {Rince,  A and Tangney,  M and Fitzgerald,  GF },
   = {2000},
   = {January},
   = {Fems Microbiology Letters},
   = {Identification of a DNA region from lactococcal phage sk1 protecting phage 712 from the abortive infection mechanism AbiF},
   = {Validated},
   = {()},
   = {small isometric-headed phage 712 AbiF abortive infection mechanism PLASMID PNP40 LACTIS BACTERIOPHAGE-SK1 RESISTANCE TRANSCRIPTION STREPTOCOCCI SEQUENCE CLONING OPERON},
   = {182},
  pages = {185--191},
   = {{Bacteriophage 712 is a small isometric-headed phage which is sensitive to the lactococcal abortive infection mechanism AbiF. Its 29.6-kb DNA genome was characterized by restriction mapping and transcriptional analysis. Construction of a gene bank of lactococcal phage ski, which is insensitive to the action of AbiF, in Lactococcus lactis containing AbiF resulted in the identification of a 324-bp DNA fragment which reduced the effect of the abortive infection mechanism on phage 712. Analysis of this region provided evidence that the action of AbiF is related to the cos ends of small isometric-headed phages. Sequence analysis of a 3.2-kb segment containing the middle operon and the cos ends of phage 712 genome allowed comparison of this part of the phage 712 genome with the equivalent sequences of four other small isometric-headed phages. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.}},
  source = {IRIS}
}
AUTHORSRince, A,Tangney, M,Fitzgerald, GF
YEAR2000
MONTHJanuary
JOURNAL_CODEFems Microbiology Letters
TITLEIdentification of a DNA region from lactococcal phage sk1 protecting phage 712 from the abortive infection mechanism AbiF
STATUSValidated
TIMES_CITED()
SEARCH_KEYWORDsmall isometric-headed phage 712 AbiF abortive infection mechanism PLASMID PNP40 LACTIS BACTERIOPHAGE-SK1 RESISTANCE TRANSCRIPTION STREPTOCOCCI SEQUENCE CLONING OPERON
VOLUME182
ISSUE
START_PAGE185
END_PAGE191
ABSTRACTBacteriophage 712 is a small isometric-headed phage which is sensitive to the lactococcal abortive infection mechanism AbiF. Its 29.6-kb DNA genome was characterized by restriction mapping and transcriptional analysis. Construction of a gene bank of lactococcal phage ski, which is insensitive to the action of AbiF, in Lactococcus lactis containing AbiF resulted in the identification of a 324-bp DNA fragment which reduced the effect of the abortive infection mechanism on phage 712. Analysis of this region provided evidence that the action of AbiF is related to the cos ends of small isometric-headed phages. Sequence analysis of a 3.2-kb segment containing the middle operon and the cos ends of phage 712 genome allowed comparison of this part of the phage 712 genome with the equivalent sequences of four other small isometric-headed phages. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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