IRIS publication 113214856
Extent of hydrolysis effects on casein hydrolysate bioactivity: Evaluation using the human Jurkat T cell line
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TY - JOUR - Lahart, Natasha and O'Callaghan, Yvonne and Aherne, S. Aisling and O'Sullivan, Dara and FitzGerald, Richard J. and O'Brien, Nora M. - 2011 - International Dairy Journal - Extent of hydrolysis effects on casein hydrolysate bioactivity: Evaluation using the human Jurkat T cell line - Validated - () - 21 - 10 - 777 - 782 - Two proteolytic preparations, A and F, identified in a previous study to yield casein hydrolysates with the highest bioactivity in Jurkat T cells, were used to hydrolyse sodium caseinate to determine if the bioactive effects of resultant hydrolysates (A1-A5 and F1-F5) were influenced by degree of hydrolysis (DH). None of the hydrolysates (0.25-10.0%, v/v) adversely affected membrane integrity. However, A4 significantly (P< 0.05) reduced Jurkat T cell viability at all concentrations and F5 decreased cell viability at concentrations exceeding 2.50% (v/v). All A hydrolysates, except A4, significantly affected both glutathione (GSH) content and cellular catalase activity at 0.5% (v/v). The effect of F hydrolysates on GSH content were variable. All samples apart from A4 significantly increased catalase activity. None of the casein hydrolysates significantly affected (P < 0.05) ConA-stimulated interleukin-2 production; however, A2 and A3 increased interleukin-10 production. While hydrolysis is considered necessary to release bioactive peptides, no direct bioactivity-DH relationship was observed. (C) 2011 Elsevier Ltd. All rights reserved. DA - 2011/NaN ER -
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@article{V113214856, = {Lahart, Natasha and O'Callaghan, Yvonne and Aherne, S. Aisling and O'Sullivan, Dara and FitzGerald, Richard J. and O'Brien, Nora M.}, = {2011}, = {International Dairy Journal}, = {Extent of hydrolysis effects on casein hydrolysate bioactivity: Evaluation using the human Jurkat T cell line}, = {Validated}, = {()}, = {21}, = {10}, pages = {777--782}, = {{Two proteolytic preparations, A and F, identified in a previous study to yield casein hydrolysates with the highest bioactivity in Jurkat T cells, were used to hydrolyse sodium caseinate to determine if the bioactive effects of resultant hydrolysates (A1-A5 and F1-F5) were influenced by degree of hydrolysis (DH). None of the hydrolysates (0.25-10.0%, v/v) adversely affected membrane integrity. However, A4 significantly (P< 0.05) reduced Jurkat T cell viability at all concentrations and F5 decreased cell viability at concentrations exceeding 2.50% (v/v). All A hydrolysates, except A4, significantly affected both glutathione (GSH) content and cellular catalase activity at 0.5% (v/v). The effect of F hydrolysates on GSH content were variable. All samples apart from A4 significantly increased catalase activity. None of the casein hydrolysates significantly affected (P < 0.05) ConA-stimulated interleukin-2 production; however, A2 and A3 increased interleukin-10 production. While hydrolysis is considered necessary to release bioactive peptides, no direct bioactivity-DH relationship was observed. (C) 2011 Elsevier Ltd. All rights reserved.}}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Lahart, Natasha and O'Callaghan, Yvonne and Aherne, S. Aisling and O'Sullivan, Dara and FitzGerald, Richard J. and O'Brien, Nora M. | ||
YEAR | 2011 | ||
MONTH | |||
JOURNAL_CODE | International Dairy Journal | ||
TITLE | Extent of hydrolysis effects on casein hydrolysate bioactivity: Evaluation using the human Jurkat T cell line | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 21 | ||
ISSUE | 10 | ||
START_PAGE | 777 | ||
END_PAGE | 782 | ||
ABSTRACT | Two proteolytic preparations, A and F, identified in a previous study to yield casein hydrolysates with the highest bioactivity in Jurkat T cells, were used to hydrolyse sodium caseinate to determine if the bioactive effects of resultant hydrolysates (A1-A5 and F1-F5) were influenced by degree of hydrolysis (DH). None of the hydrolysates (0.25-10.0%, v/v) adversely affected membrane integrity. However, A4 significantly (P< 0.05) reduced Jurkat T cell viability at all concentrations and F5 decreased cell viability at concentrations exceeding 2.50% (v/v). All A hydrolysates, except A4, significantly affected both glutathione (GSH) content and cellular catalase activity at 0.5% (v/v). The effect of F hydrolysates on GSH content were variable. All samples apart from A4 significantly increased catalase activity. None of the casein hydrolysates significantly affected (P < 0.05) ConA-stimulated interleukin-2 production; however, A2 and A3 increased interleukin-10 production. While hydrolysis is considered necessary to release bioactive peptides, no direct bioactivity-DH relationship was observed. (C) 2011 Elsevier Ltd. All rights reserved. | ||
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