IRIS publication 113214893
gamma-tocopherol is less effective than alpha-tocopherol in preventing oxidant-induced sister chromatid exchanges in Chinese hamster V79 cells
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TY - JOUR - O'Leary, K. A. and Woods, J. A. and O'Brien, N. M. - 2001 - Free Radical Research - gamma-tocopherol is less effective than alpha-tocopherol in preventing oxidant-induced sister chromatid exchanges in Chinese hamster V79 cells - Validated - () - 35 - 6 - 917 - 924 - Although alpha-tocopherol (alpha-TOC) is the most biologically active form of vitamin E and is found at high levels in plasma, gamma-tocopherol (gamma-TOC) has also been found to be a powerful antioxidant in vitro and constitutes up to 70% of the dietary intake of TOC. Low plasma levels of gamma-TOC and a high alpha-TOC:gamma-TOC ratio may be associated with coronary heart disease, suggesting that there may be a positive protective role for the gamma-form of TOC. In this study the ability of different forms of vitamin E to protect against sister chromatid exchanges (SCE) induced by either hydrogen peroxide or menadione was investigated. Chinese hamster V79 cells were pre-treated with 10 muM TOC for 24h, and then challenged with a genotoxin. After a 24h pre-treatment, there was a I eater incorporation of gamma-TOC (319.8 +/- 66.2mg/10(6) cells) into V79 cells compared to alpha-TOC (66.9 +/- 6.4ng/10(6) cells). gamma-TOC did not protect the cells against SCE induced by either hydrogen peroxide or menadione, alpha-TOC acetate was partially protective against both genotoxins, whereas alpha-TOC completely abolished the oxidant induced SCE. These results demonstrate that, despite a greater incorporation of gamma-TOC into V79 cells, alpha-TOC but not gamma-TOC was more effective at inhibiting oxidatively-induced SCE in V79 cells. DA - 2001/NaN ER -
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@article{V113214893, = {O'Leary, K. A. and Woods, J. A. and O'Brien, N. M.}, = {2001}, = {Free Radical Research}, = {gamma-tocopherol is less effective than alpha-tocopherol in preventing oxidant-induced sister chromatid exchanges in Chinese hamster V79 cells}, = {Validated}, = {()}, = {35}, = {6}, pages = {917--924}, = {{Although alpha-tocopherol (alpha-TOC) is the most biologically active form of vitamin E and is found at high levels in plasma, gamma-tocopherol (gamma-TOC) has also been found to be a powerful antioxidant in vitro and constitutes up to 70% of the dietary intake of TOC. Low plasma levels of gamma-TOC and a high alpha-TOC:gamma-TOC ratio may be associated with coronary heart disease, suggesting that there may be a positive protective role for the gamma-form of TOC. In this study the ability of different forms of vitamin E to protect against sister chromatid exchanges (SCE) induced by either hydrogen peroxide or menadione was investigated. Chinese hamster V79 cells were pre-treated with 10 muM TOC for 24h, and then challenged with a genotoxin. After a 24h pre-treatment, there was a I eater incorporation of gamma-TOC (319.8 +/- 66.2mg/10(6) cells) into V79 cells compared to alpha-TOC (66.9 +/- 6.4ng/10(6) cells). gamma-TOC did not protect the cells against SCE induced by either hydrogen peroxide or menadione, alpha-TOC acetate was partially protective against both genotoxins, whereas alpha-TOC completely abolished the oxidant induced SCE. These results demonstrate that, despite a greater incorporation of gamma-TOC into V79 cells, alpha-TOC but not gamma-TOC was more effective at inhibiting oxidatively-induced SCE in V79 cells.}}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | O'Leary, K. A. and Woods, J. A. and O'Brien, N. M. | ||
YEAR | 2001 | ||
MONTH | |||
JOURNAL_CODE | Free Radical Research | ||
TITLE | gamma-tocopherol is less effective than alpha-tocopherol in preventing oxidant-induced sister chromatid exchanges in Chinese hamster V79 cells | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 35 | ||
ISSUE | 6 | ||
START_PAGE | 917 | ||
END_PAGE | 924 | ||
ABSTRACT | Although alpha-tocopherol (alpha-TOC) is the most biologically active form of vitamin E and is found at high levels in plasma, gamma-tocopherol (gamma-TOC) has also been found to be a powerful antioxidant in vitro and constitutes up to 70% of the dietary intake of TOC. Low plasma levels of gamma-TOC and a high alpha-TOC:gamma-TOC ratio may be associated with coronary heart disease, suggesting that there may be a positive protective role for the gamma-form of TOC. In this study the ability of different forms of vitamin E to protect against sister chromatid exchanges (SCE) induced by either hydrogen peroxide or menadione was investigated. Chinese hamster V79 cells were pre-treated with 10 muM TOC for 24h, and then challenged with a genotoxin. After a 24h pre-treatment, there was a I eater incorporation of gamma-TOC (319.8 +/- 66.2mg/10(6) cells) into V79 cells compared to alpha-TOC (66.9 +/- 6.4ng/10(6) cells). gamma-TOC did not protect the cells against SCE induced by either hydrogen peroxide or menadione, alpha-TOC acetate was partially protective against both genotoxins, whereas alpha-TOC completely abolished the oxidant induced SCE. These results demonstrate that, despite a greater incorporation of gamma-TOC into V79 cells, alpha-TOC but not gamma-TOC was more effective at inhibiting oxidatively-induced SCE in V79 cells. | ||
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