IRIS publication 113214900
Investigation of beta-carotene and Lutein Transport in Caco-2 Cells: Carotenoid-carotenoid Interactions and Transport Inhibition by Ezetimibe
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TY - JOUR - O'Sullivan, Laurie and Aherne, S. Aisling and O'Brien, Nora M. - 2009 - International Journal For Vitamin and Nutrition Research - Investigation of beta-carotene and Lutein Transport in Caco-2 Cells: Carotenoid-carotenoid Interactions and Transport Inhibition by Ezetimibe - Validated - () - 79 - 5-6 - 337 - 347 - Carotenoid bioavailability is influenced by a number of factors including the presence of other carotenoids, which may enhance or inhibit the transport of one another by intestinal cells. Therefore, the objectives of the present study were: first, to determine carotenoid uptake and secretion (i.e. transport) by supplementing differentiated Caco-2 cells with increasing concentrations of either lutein or beta-carotene (0-1 mu M.); second, to assess any interactions between beta-carotene and lutein on their cellular uptake and secretion; and third, to a minor extent, to determine the effects of a carotenoid absorption inhibitor, ezetimibe, on beta-carotene and lutein transport. The carotenoid mixes were used at molar ratios of 1:1 and 4:1. At equimolar concentrations, lutein had a negative impact on beta-carotene transport and vice versa. However, these effects were not seen when the ratios were adjusted to 4:1. Following treatment with ezetimibe (25-100 mu M) for 16 hours there was a reduction in beta-carotene transport, whereas a non-significant reduction in lutein transport was observed. In conclusion, this study confirmed that beta-carotene and lutein interact during their absorption, depending on the concentration/ratios used, and that carotenoid absorption is partially affected by ezetimibe. DA - 2009/NaN ER -
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@article{V113214900, = {O'Sullivan, Laurie and Aherne, S. Aisling and O'Brien, Nora M.}, = {2009}, = {International Journal For Vitamin and Nutrition Research}, = {Investigation of beta-carotene and Lutein Transport in Caco-2 Cells: Carotenoid-carotenoid Interactions and Transport Inhibition by Ezetimibe}, = {Validated}, = {()}, = {79}, = {5-6}, pages = {337--347}, = {{Carotenoid bioavailability is influenced by a number of factors including the presence of other carotenoids, which may enhance or inhibit the transport of one another by intestinal cells. Therefore, the objectives of the present study were: first, to determine carotenoid uptake and secretion (i.e. transport) by supplementing differentiated Caco-2 cells with increasing concentrations of either lutein or beta-carotene (0-1 mu M.); second, to assess any interactions between beta-carotene and lutein on their cellular uptake and secretion; and third, to a minor extent, to determine the effects of a carotenoid absorption inhibitor, ezetimibe, on beta-carotene and lutein transport. The carotenoid mixes were used at molar ratios of 1:1 and 4:1. At equimolar concentrations, lutein had a negative impact on beta-carotene transport and vice versa. However, these effects were not seen when the ratios were adjusted to 4:1. Following treatment with ezetimibe (25-100 mu M) for 16 hours there was a reduction in beta-carotene transport, whereas a non-significant reduction in lutein transport was observed. In conclusion, this study confirmed that beta-carotene and lutein interact during their absorption, depending on the concentration/ratios used, and that carotenoid absorption is partially affected by ezetimibe.}}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | O'Sullivan, Laurie and Aherne, S. Aisling and O'Brien, Nora M. | ||
YEAR | 2009 | ||
MONTH | |||
JOURNAL_CODE | International Journal For Vitamin and Nutrition Research | ||
TITLE | Investigation of beta-carotene and Lutein Transport in Caco-2 Cells: Carotenoid-carotenoid Interactions and Transport Inhibition by Ezetimibe | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 79 | ||
ISSUE | 5-6 | ||
START_PAGE | 337 | ||
END_PAGE | 347 | ||
ABSTRACT | Carotenoid bioavailability is influenced by a number of factors including the presence of other carotenoids, which may enhance or inhibit the transport of one another by intestinal cells. Therefore, the objectives of the present study were: first, to determine carotenoid uptake and secretion (i.e. transport) by supplementing differentiated Caco-2 cells with increasing concentrations of either lutein or beta-carotene (0-1 mu M.); second, to assess any interactions between beta-carotene and lutein on their cellular uptake and secretion; and third, to a minor extent, to determine the effects of a carotenoid absorption inhibitor, ezetimibe, on beta-carotene and lutein transport. The carotenoid mixes were used at molar ratios of 1:1 and 4:1. At equimolar concentrations, lutein had a negative impact on beta-carotene transport and vice versa. However, these effects were not seen when the ratios were adjusted to 4:1. Following treatment with ezetimibe (25-100 mu M) for 16 hours there was a reduction in beta-carotene transport, whereas a non-significant reduction in lutein transport was observed. In conclusion, this study confirmed that beta-carotene and lutein interact during their absorption, depending on the concentration/ratios used, and that carotenoid absorption is partially affected by ezetimibe. | ||
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