TY  - JOUR
  - Hartl, MGJ,Kilemade, M,Sheehan, D,Mothersill, C,O'Halloran, J,O'Brien, NM,van Pelt, FNAM
  - 2007
  - July
  - Marine Environmental Research
  - Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.
  - Validated
  - ()
  - sediment cytochrome P450 western blotting comet assay pleuronectiformes FLOUNDER PLATICHTHYS-FLESUS ALKALINE COMET ASSAY DNA STRAND BREAKS PLAICE PLEURONECTES-PLATESSA RAINBOW-TROUT HEPATOCYTES CELL GEL-ELECTROPHORESIS GADUS-MORHUA L ATLANTIC COD AROMATIC-HYDROCARBONS CHRONIC EXPOSURE
  - 64
  - 191
  - 208
  - Hatchery-reared turbot (Scophthalmus maximus L.) were exposed for 3 weeks, under laboratory conditions, to sediment collected from polluted sites in Cork Harbour and a reference site at Ballymacoda, Co. Cork, Ireland. The potential of surficial sediment for inducing hepatic biomarkers was assessed at two levels of biological organisation: expression of cytochrome P450 [Western blotting analysis and 7-ethoxy-resorufin O-dealkylase (EROD), 7-benzoxy resorufin O-dealkylase (BROD), 7-methoxy resorufin O-dealkylase (MROD), 7-pentoxy-resorufin O-dealkylase (PROD) activities] and DNA integrity (Comet assay). Positive controls were generated, either by exposing turbot to cadmium chloride-spiked seawater (Comet assay) or to beta-naphthaflavone by intraperitoneal injection (cytochrome P450 induction). The induction of cytochrome P450 activity (EROD, MROD and PROD) in animals following a 7-day exposure to contaminated sediments was significantly higher than those exposed to reference site sediment and remained elevated thereafter; BROD was not induced. DNA single-strand breaks were also significantly higher following exposure to contaminated sediments throughout the experiment. Although no direct correlation between induction of alkoxyresorufin O-dealkylase activities and a particular chemical class was established, the induction of MROD and PROD activities in fish exposed to sediments containing complex contaminant mixtures, appeared to be more sensitive than conventional EROD activity assays. We conclude from the present laboratory study that S. maximus is a suitable sentinel species for the assessment of moderately contaminated sediments and therefore allows for the further development of this model for future, ecologically relevant, field studies. (c) 2007 Elsevier Ltd. All rights reserved.
  - DOI 10.1016/j.marenvres.2007.01.002
DA  - 2007/07
ER  - 

@article{V43336010,
   = {Hartl,  MGJ and Kilemade,  M and Sheehan,  D and Mothersill,  C and O'Halloran,  J and O'Brien,  NM and van Pelt,  FNAM },
   = {2007},
   = {July},
   = {Marine Environmental Research},
   = {Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.},
   = {Validated},
   = {()},
   = {sediment cytochrome P450 western blotting comet assay pleuronectiformes FLOUNDER PLATICHTHYS-FLESUS ALKALINE COMET ASSAY DNA STRAND BREAKS PLAICE PLEURONECTES-PLATESSA RAINBOW-TROUT HEPATOCYTES CELL GEL-ELECTROPHORESIS GADUS-MORHUA L ATLANTIC COD AROMATIC-HYDROCARBONS CHRONIC EXPOSURE},
   = {64},
  pages = {191--208},
   = {{Hatchery-reared turbot (Scophthalmus maximus L.) were exposed for 3 weeks, under laboratory conditions, to sediment collected from polluted sites in Cork Harbour and a reference site at Ballymacoda, Co. Cork, Ireland. The potential of surficial sediment for inducing hepatic biomarkers was assessed at two levels of biological organisation: expression of cytochrome P450 [Western blotting analysis and 7-ethoxy-resorufin O-dealkylase (EROD), 7-benzoxy resorufin O-dealkylase (BROD), 7-methoxy resorufin O-dealkylase (MROD), 7-pentoxy-resorufin O-dealkylase (PROD) activities] and DNA integrity (Comet assay). Positive controls were generated, either by exposing turbot to cadmium chloride-spiked seawater (Comet assay) or to beta-naphthaflavone by intraperitoneal injection (cytochrome P450 induction). The induction of cytochrome P450 activity (EROD, MROD and PROD) in animals following a 7-day exposure to contaminated sediments was significantly higher than those exposed to reference site sediment and remained elevated thereafter; BROD was not induced. DNA single-strand breaks were also significantly higher following exposure to contaminated sediments throughout the experiment. Although no direct correlation between induction of alkoxyresorufin O-dealkylase activities and a particular chemical class was established, the induction of MROD and PROD activities in fish exposed to sediments containing complex contaminant mixtures, appeared to be more sensitive than conventional EROD activity assays. We conclude from the present laboratory study that S. maximus is a suitable sentinel species for the assessment of moderately contaminated sediments and therefore allows for the further development of this model for future, ecologically relevant, field studies. (c) 2007 Elsevier Ltd. All rights reserved.}},
   = {DOI 10.1016/j.marenvres.2007.01.002},
  source = {IRIS}
}