IRIS publication 43337945
Toxicity of cholesterol oxidation products to Caco-2 and HepG2 cells: Modulatory effects of alpha- and gamma-tocopherol
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TY - JOUR - O'Sullivan, AJ,O'Callaghan, YC,Woods, JA,O'Brien', NM - 2003 - March - Rna-A Publication of The Rna Society - Toxicity of cholesterol oxidation products to Caco-2 and HepG2 cells: Modulatory effects of alpha- and gamma-tocopherol - Validated - () - Caco-2 cells HepG2 cells oxysterol alpha-tocopherol gamma-tocopherol SMOOTH-MUSCLE CELLS A REDUCTASE-ACTIVITY VITAMIN-E ENDOTHELIAL-CELLS INDUCED APOPTOSIS OXYSTEROLS CYTOTOXICITY 25-HYDROXYCHOLESTEROL TOCOTRIENOLS DERIVATIVES - 23 - 191 - 197 - Cholesterol can be oxidized to form a variety of cholesterol oxidation products also known as oxysterols. The aims of the present study were to compare the cytotoxic effects of four oxysterols, namely 25-hydroxycholesterol (25-OHC), 7beta-hydroxycholesterol (7beta-OHC), cholesterol-5beta,6beta-epoxide (beta-epox) and cholesterol-5alpha,6alpha-epoxide (alpha-epox), in two human cell culture models. Further, the ability of 10 and 100 mum alpha- and gamma-tocopherol (alpha-TOC and gamma-TOC, respectively) to protect against oxysterol-induced cytotoxicity was also assessed. Human colonic adenocarcinoma Caco-2 and human hepatoma HepG2 cells were supplemented with increasing concentrations of 25-OHC, 7beta-OHC, beta-epox and alpha-epox (0-25 mug ml(-1)) for 24,48 or 96 h. Following 24-h and 48-h exposure, test media were replaced with normal growth media and the cells were maintained for 72 and 48 h, respectively. The 96-h exposure represented a constant challenge to the cells. Cytotoxicity was assessed using the neutral red uptake assay. The concentration of compound that inhibited cell viability by 50% (IC50 value) was calculated. All four oxysterols investigated induced the greatest cytotoxic effects following 96 It of exposure. 25-Hydroxycholesterol exhibited the greatest cytotoxicity in both cell lines. Both beta-epox and alpha-epox were more toxic to HepG2 cells than to Caco-2 cells after the 48-h exposure. Pretreatment of cells with either alpha- or gamma-TOC did not protect against oxysterol-induced cytotoxicity. The Caco-2 cells treated with the high concentration (100 mum) of gamma-TOC were found to be more susceptible to oxysterol-induced toxicity under the conditions employed in this study. Copyright (C) 2003 John Wiley Sons, Ltd. - DOI 10.1002/jat.906 DA - 2003/03 ER -
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@article{V43337945, = {O'Sullivan, AJ and O'Callaghan, YC and Woods, JA and O'Brien', NM }, = {2003}, = {March}, = {Rna-A Publication of The Rna Society}, = {Toxicity of cholesterol oxidation products to Caco-2 and HepG2 cells: Modulatory effects of alpha- and gamma-tocopherol}, = {Validated}, = {()}, = {Caco-2 cells HepG2 cells oxysterol alpha-tocopherol gamma-tocopherol SMOOTH-MUSCLE CELLS A REDUCTASE-ACTIVITY VITAMIN-E ENDOTHELIAL-CELLS INDUCED APOPTOSIS OXYSTEROLS CYTOTOXICITY 25-HYDROXYCHOLESTEROL TOCOTRIENOLS DERIVATIVES}, = {23}, pages = {191--197}, = {{Cholesterol can be oxidized to form a variety of cholesterol oxidation products also known as oxysterols. The aims of the present study were to compare the cytotoxic effects of four oxysterols, namely 25-hydroxycholesterol (25-OHC), 7beta-hydroxycholesterol (7beta-OHC), cholesterol-5beta,6beta-epoxide (beta-epox) and cholesterol-5alpha,6alpha-epoxide (alpha-epox), in two human cell culture models. Further, the ability of 10 and 100 mum alpha- and gamma-tocopherol (alpha-TOC and gamma-TOC, respectively) to protect against oxysterol-induced cytotoxicity was also assessed. Human colonic adenocarcinoma Caco-2 and human hepatoma HepG2 cells were supplemented with increasing concentrations of 25-OHC, 7beta-OHC, beta-epox and alpha-epox (0-25 mug ml(-1)) for 24,48 or 96 h. Following 24-h and 48-h exposure, test media were replaced with normal growth media and the cells were maintained for 72 and 48 h, respectively. The 96-h exposure represented a constant challenge to the cells. Cytotoxicity was assessed using the neutral red uptake assay. The concentration of compound that inhibited cell viability by 50% (IC50 value) was calculated. All four oxysterols investigated induced the greatest cytotoxic effects following 96 It of exposure. 25-Hydroxycholesterol exhibited the greatest cytotoxicity in both cell lines. Both beta-epox and alpha-epox were more toxic to HepG2 cells than to Caco-2 cells after the 48-h exposure. Pretreatment of cells with either alpha- or gamma-TOC did not protect against oxysterol-induced cytotoxicity. The Caco-2 cells treated with the high concentration (100 mum) of gamma-TOC were found to be more susceptible to oxysterol-induced toxicity under the conditions employed in this study. Copyright (C) 2003 John Wiley Sons, Ltd.}}, = {DOI 10.1002/jat.906}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | O'Sullivan, AJ,O'Callaghan, YC,Woods, JA,O'Brien', NM | ||
YEAR | 2003 | ||
MONTH | March | ||
JOURNAL_CODE | Rna-A Publication of The Rna Society | ||
TITLE | Toxicity of cholesterol oxidation products to Caco-2 and HepG2 cells: Modulatory effects of alpha- and gamma-tocopherol | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | Caco-2 cells HepG2 cells oxysterol alpha-tocopherol gamma-tocopherol SMOOTH-MUSCLE CELLS A REDUCTASE-ACTIVITY VITAMIN-E ENDOTHELIAL-CELLS INDUCED APOPTOSIS OXYSTEROLS CYTOTOXICITY 25-HYDROXYCHOLESTEROL TOCOTRIENOLS DERIVATIVES | ||
VOLUME | 23 | ||
ISSUE | |||
START_PAGE | 191 | ||
END_PAGE | 197 | ||
ABSTRACT | Cholesterol can be oxidized to form a variety of cholesterol oxidation products also known as oxysterols. The aims of the present study were to compare the cytotoxic effects of four oxysterols, namely 25-hydroxycholesterol (25-OHC), 7beta-hydroxycholesterol (7beta-OHC), cholesterol-5beta,6beta-epoxide (beta-epox) and cholesterol-5alpha,6alpha-epoxide (alpha-epox), in two human cell culture models. Further, the ability of 10 and 100 mum alpha- and gamma-tocopherol (alpha-TOC and gamma-TOC, respectively) to protect against oxysterol-induced cytotoxicity was also assessed. Human colonic adenocarcinoma Caco-2 and human hepatoma HepG2 cells were supplemented with increasing concentrations of 25-OHC, 7beta-OHC, beta-epox and alpha-epox (0-25 mug ml(-1)) for 24,48 or 96 h. Following 24-h and 48-h exposure, test media were replaced with normal growth media and the cells were maintained for 72 and 48 h, respectively. The 96-h exposure represented a constant challenge to the cells. Cytotoxicity was assessed using the neutral red uptake assay. The concentration of compound that inhibited cell viability by 50% (IC50 value) was calculated. All four oxysterols investigated induced the greatest cytotoxic effects following 96 It of exposure. 25-Hydroxycholesterol exhibited the greatest cytotoxicity in both cell lines. Both beta-epox and alpha-epox were more toxic to HepG2 cells than to Caco-2 cells after the 48-h exposure. Pretreatment of cells with either alpha- or gamma-TOC did not protect against oxysterol-induced cytotoxicity. The Caco-2 cells treated with the high concentration (100 mum) of gamma-TOC were found to be more susceptible to oxysterol-induced toxicity under the conditions employed in this study. Copyright (C) 2003 John Wiley Sons, Ltd. | ||
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DOI_LINK | DOI 10.1002/jat.906 | ||
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