IRIS publication 720050
Lack of Genoprotective Effect of Phytosterols and Conjugated Linoleic Acids On Caco-2 Cells
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TY - JOUR - Daly, TJ, Aherne, SA, O'Connor, TP, O'Brien, NM - 2009 - August - Food and Chemical Toxicology - Lack of Genoprotective Effect of Phytosterols and Conjugated Linoleic Acids On Caco-2 Cells - Validated - () - 47 - 8 - 1791 - 1796 - Much interest has focused on the cholesterol-lowering effects of phytosterols (plant sterols) but limited data suggests they may also possess anti-carcinogenic activity. Conjugated linoleic acids (CLA), sourced from meat and dairy products of ruminant animals, has also received considerable attention as a potential anti-cancer agent. Therefore, the aims of this project were to (i) examine the effects of phytosterols and CLA on the viability and growth of human intestinal Caco-2 cells and (ii) determine their potential genoprotective (comet assay), COX-2 modulatory (ELISA) and apoptotic (Hoechst staining) activities. Caco-2 cells were supplemented with the phytosterols campesterol, beta-sitosterol, or beta-sitostanot, or a CLA mixture, or individual CLA isomers (c10t12-CLA, t9t11-CLA) for 48 h. The three phytosterols, at the highest levels tested, were found to reduce both the viability and growth of Caco-2 cells while CIA exhibited isomer-specific effects. None of the phytorsterols protected against DNA damage. At a concentration of 25 mu M, both c10t12-CLA and t9t11-CLA enhanced (P < 0.05) oxidant-induced, but not mutagen-induced, DNA damage. Neither the phytosterols nor CIA induced apoptosis or modulated COX-2 production. In conclusion, campesterol, beta-sitosterol, beta-sitostanol, c10t12-CLA, and t9t11-CLA were not toxic to Caco-2 cells, at the lower levels tested, and did not exhibit potential anti-carcinogenic activity. (C) 2009 Elsevier Ltd. All rights reserved.. - DOI 10.1016/j.fct.2009.04.031 DA - 2009/08 ER -
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@article{V720050, = {Daly, TJ and Aherne, SA and O'Connor, TP and O'Brien, NM }, = {2009}, = {August}, = {Food and Chemical Toxicology}, = {Lack of Genoprotective Effect of Phytosterols and Conjugated Linoleic Acids On Caco-2 Cells}, = {Validated}, = {()}, = {47}, = {8}, pages = {1791--1796}, = {{Much interest has focused on the cholesterol-lowering effects of phytosterols (plant sterols) but limited data suggests they may also possess anti-carcinogenic activity. Conjugated linoleic acids (CLA), sourced from meat and dairy products of ruminant animals, has also received considerable attention as a potential anti-cancer agent. Therefore, the aims of this project were to (i) examine the effects of phytosterols and CLA on the viability and growth of human intestinal Caco-2 cells and (ii) determine their potential genoprotective (comet assay), COX-2 modulatory (ELISA) and apoptotic (Hoechst staining) activities. Caco-2 cells were supplemented with the phytosterols campesterol, beta-sitosterol, or beta-sitostanot, or a CLA mixture, or individual CLA isomers (c10t12-CLA, t9t11-CLA) for 48 h. The three phytosterols, at the highest levels tested, were found to reduce both the viability and growth of Caco-2 cells while CIA exhibited isomer-specific effects. None of the phytorsterols protected against DNA damage. At a concentration of 25 mu M, both c10t12-CLA and t9t11-CLA enhanced (P < 0.05) oxidant-induced, but not mutagen-induced, DNA damage. Neither the phytosterols nor CIA induced apoptosis or modulated COX-2 production. In conclusion, campesterol, beta-sitosterol, beta-sitostanol, c10t12-CLA, and t9t11-CLA were not toxic to Caco-2 cells, at the lower levels tested, and did not exhibit potential anti-carcinogenic activity. (C) 2009 Elsevier Ltd. All rights reserved..}}, = {DOI 10.1016/j.fct.2009.04.031}, source = {IRIS} }
Data as stored in IRIS
AUTHORS | Daly, TJ, Aherne, SA, O'Connor, TP, O'Brien, NM | ||
YEAR | 2009 | ||
MONTH | August | ||
JOURNAL_CODE | Food and Chemical Toxicology | ||
TITLE | Lack of Genoprotective Effect of Phytosterols and Conjugated Linoleic Acids On Caco-2 Cells | ||
STATUS | Validated | ||
TIMES_CITED | () | ||
SEARCH_KEYWORD | |||
VOLUME | 47 | ||
ISSUE | 8 | ||
START_PAGE | 1791 | ||
END_PAGE | 1796 | ||
ABSTRACT | Much interest has focused on the cholesterol-lowering effects of phytosterols (plant sterols) but limited data suggests they may also possess anti-carcinogenic activity. Conjugated linoleic acids (CLA), sourced from meat and dairy products of ruminant animals, has also received considerable attention as a potential anti-cancer agent. Therefore, the aims of this project were to (i) examine the effects of phytosterols and CLA on the viability and growth of human intestinal Caco-2 cells and (ii) determine their potential genoprotective (comet assay), COX-2 modulatory (ELISA) and apoptotic (Hoechst staining) activities. Caco-2 cells were supplemented with the phytosterols campesterol, beta-sitosterol, or beta-sitostanot, or a CLA mixture, or individual CLA isomers (c10t12-CLA, t9t11-CLA) for 48 h. The three phytosterols, at the highest levels tested, were found to reduce both the viability and growth of Caco-2 cells while CIA exhibited isomer-specific effects. None of the phytorsterols protected against DNA damage. At a concentration of 25 mu M, both c10t12-CLA and t9t11-CLA enhanced (P < 0.05) oxidant-induced, but not mutagen-induced, DNA damage. Neither the phytosterols nor CIA induced apoptosis or modulated COX-2 production. In conclusion, campesterol, beta-sitosterol, beta-sitostanol, c10t12-CLA, and t9t11-CLA were not toxic to Caco-2 cells, at the lower levels tested, and did not exhibit potential anti-carcinogenic activity. (C) 2009 Elsevier Ltd. All rights reserved.. | ||
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DOI_LINK | DOI 10.1016/j.fct.2009.04.031 | ||
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