My scientific research is focussed upon cellular signalling in central neurons utilising whole-cell patch clamp electrophysiology combined with intracellular calcium imaging. This work is aimed at developing our understanding of the mechanisms underlying learning and memory, both in health and in conditions such as Duchenne muscular dystrophy and Alzheimer's disease where cognition and/or memory are adversely affected.
My primary research focusses on the role that intracellular calcium signalling (e.g. through activation of endoplasmic reticulum IP3 and ryanodine receptors) plays in hippocampal neuronal signalling. Initial work in this area demonstrated that group I metabotropic receptors (I-mGluRs) could potentially act as a novel form of coincidence detector within the CNS, a role that previously been ascribed to NMDA receptors alone (Rae et al., 2000). Further work clarified the relative contribution of the two subtypes of I-mGluRs expressed within hippocampal neurons to the calcium and electrical signals generated by stimulation of these receptors (Rae & Irving, 2004).
Based upon current pedagogy, I have trialled/adopted various teaching methodologies since 2013, all of which have been geared towards increasing active student participation during class time, with a view to increasing the understanding and application of taught material. These methodologies include:
Producing pre-recorded short introductory, and full lecture length, vodcasts (using Panopto®) of lecture material for students to view both before and after (for review purposes) attending lectures (see Rae & McCarthy, 2017; Rae & O’Malley, 2016 & 2017).
Pre-lecture quizzes (posted on Blackboard) to provide ‘first pass’ exposure to lecture topics prior to attending class. ‘Semi-flipped’ classroom teaching, whereby class time is used to interrogate students’ knowledge (i.e. through discussion, application, questioning, etc.) of material they have been asked to study prior to attending class.
Have integrated lectures with Socrative®, an online student response system with which students can anonymously answer questions posed by me using their smartphones or tablets. The percentage of correct (and incorrect) answers is displayed in real time allowing the provision of immediate feedback to the class.
Biography
I was awarded a B.Sc. (Hons) in Pharmacology at the University of Aberdeen and a Ph.D. in smooth muscle Physiology from the University of Glasgow. After a short period at the University of Strathclyde working with Dr. Charles Kennedy to investigate the putative role of purines in pain signalling, I then moved to Reno, Nevada, U.S.A. for two years to work with Dr. Kathy Keef investigating various aspects of smooth muscle physiology using human, canine and murine gastrointestinal tissue. Upon returning to Scotland to work with Dr. Andy Irving in both Aberdeen and Dundee University, I utilised simultaneous electrophysiological and imaging techniques to determine the function of metabotropic glutamate receptor signalling in central neurons. Prior to joining UCC I spent nearly three years in industry using my accumulated electrophysiological and calcium imaging expertise to characterise potential novel pharmaceutical solutions for the treatment of neuropathic (centrally-mediated) pain. I was appointed as a lecturer in Physiology at University College Cork in October 2008. Research Interests My primary research interest is investigating the intracellular mechanisms underlying metabotropic glutamatergic and muscarinic receptor signalling in central neurons. These receptors are of interest because of their proposed role in learning and memory as well as in neurodegenerative conditions such as Alzheimer’s Disease and Down Syndrome. Both receptor types perform an essential role in modulating intracellular calcium levels in many diverse cell types. Because calcium plays such a critical role in the normal functioning of almost every cell in the body, both as a signalling molecule and as a vital regulatory intracellular ion, it is essential that we increase our understanding of how calcium-modulating receptors such as metabotropic glutamate and muscarinic receptors affect these functions, and equally, what happens when calcium regulation goes awry. My research in this area is facilitated principally by the use of whole-cell patch clamp electrophysiological recording, whilst simultaneously measuring intracellular ion levels using fluorescent imaging techniques. These methodologies are complemented by a variety of molecular approaches that are used to investigate factors such as receptor expression and regulation within central neurons.
I currently supervise two PhD students and several undergraduate students.In 2016 I graduated with a first class Masters degree in Teaching and Learning in Higher Education. Through this, in addition to my scientific research, I have developed an interest in pedagogy (the theory and practice of education) and in 2017 was appointed as a Fellow to the Centre for the Integration of Research, Teaching and Learning (CIRTL). I am specifically interested in utilising modern technology to facilitate student understanding and learning.
Research Interests
ScientificMy scientific research is focussed upon cellular signalling in central neurons utilising whole-cell patch clamp electrophysiology combined with intracellular calcium imaging. This work is aimed at developing our understanding of the mechanisms underlying learning and memory, both in health and in conditions such as Duchenne muscular dystrophy and Alzheimer's disease where cognition and/or memory are adversely affected.
My primary research focusses on the role that intracellular calcium signalling (e.g. through activation of endoplasmic reticulum IP3 and ryanodine receptors) plays in hippocampal neuronal signalling. Initial work in this area demonstrated that group I metabotropic receptors (I-mGluRs) could potentially act as a novel form of coincidence detector within the CNS, a role that previously been ascribed to NMDA receptors alone (Rae et al., 2000). Further work clarified the relative contribution of the two subtypes of I-mGluRs expressed within hippocampal neurons to the calcium and electrical signals generated by stimulation of these receptors (Rae & Irving, 2004).
My work has sought to investigate the role that neuronal calcium dysregulation plays in the aetiology of Alzheimer's disease (using models of the familial form of the disease). In pursuit of this, we have shown that calcium signalling processes such as those mediated by I-mGluRs and cyclic ADP ribose production (leading to activation of RyRs on the ER), as well as store-operated calcium entry are significantly disrupted in transgenic hippocampal neurons relative to control neurons.
More recently, supported by an Eli-Lilly scholarship award, we have begun to investigate AD-mediated molecular processes in, arguably, a much more physiologically-relevant experimental model, the 3D cerebroid, or "mini-brain", which are grown from human pluripotent stem cells.
Additionally, using a combination of behavioural, electrophysiological and biochemical techniques, and in collaboration with Dr Dervla O'Malley (Physiology, UCC), we are also currently investigating the molecular processes underlying the poorly understood cognitive dysfunction seen in boys with Duchenne muscular dystrophy.
For these, and previous projects, I have participated in a number of research collaborations (both within and outwith UCC) investigating, for example, biochemical factors which may cause the disrupted calcium signalling we observe in transgenic Alzheimer's disease neurons (Geert Bultynck, Belgium), electrophysiological responses of enteric neurons in irritable bowel syndrome (Dervla O'Malley, UCC) and the effect of AD patient-derived cerebrospinal fluid on biomarkers of AD in human cerebroids (Drs Suzanne Timmons (UCC) & Prof. Sean Kennelly (Tallaght Hospital, Dublin)).
Teaching
An emerging area of research for me is the interrogation of my own teaching as an act of scholarship.Based upon current pedagogy, I have trialled/adopted various teaching methodologies since 2013, all of which have been geared towards increasing active student participation during class time, with a view to increasing the understanding and application of taught material. These methodologies include:
Producing pre-recorded short introductory, and full lecture length, vodcasts (using Panopto®) of lecture material for students to view both before and after (for review purposes) attending lectures (see Rae & McCarthy, 2017; Rae & O’Malley, 2016 & 2017).
Pre-lecture quizzes (posted on Blackboard) to provide ‘first pass’ exposure to lecture topics prior to attending class. ‘Semi-flipped’ classroom teaching, whereby class time is used to interrogate students’ knowledge (i.e. through discussion, application, questioning, etc.) of material they have been asked to study prior to attending class.
Have integrated lectures with Socrative®, an online student response system with which students can anonymously answer questions posed by me using their smartphones or tablets. The percentage of correct (and incorrect) answers is displayed in real time allowing the provision of immediate feedback to the class.
Research Grants
| Project | Funding Body | Start Date | End Date | Award | |
|---|---|---|---|---|---|
| Does Bexarotene reverse calcuim dysregulation in a murine model of Alzheimer's disease. | Foreign Research Institute | 26-FEB-13 | 25-FEB-14 | €11,325.00 | |
| Eli Lilly PhD Scholarship (Michael Vaughan) | Industry | 01-OCT-18 | 30-SEP-22 | €112,000.00 | |
| How does cerebrospinal fluid from Alzheimer’s disease patients affect human cortical forebrain cerebroid form and function? | College of Medicine & Health | 14-OCT-19 | 14-OCT-20 | €10,000.00 | |
| Electrophysiological studies on the F344-AD rat | UCC Strategic Research Fund | 09-AUG-14 | €4,700.00 | ||
| HRB - Is Down Syndrome a form of "calciumopathy"? | Health Research Board | 08-JUN-09 | 31-JUL-09 | €2,000.00 | |
| A novel rat model of Alzheimer's disease, the F344-AD rat | UCC Strategic Research Fund | 01-JUL-13 | €5,300.00 | ||
| UCC"Strategic Research Fund 2014" | Miscellaneous | 01-SEP-14 | 31-AUG-15 | €4,700.00 |
Publications
Peer Reviewed Journals
| Year | Publication | |
|---|---|---|
| (2019) | 'How much do preclinical medical students utilize the internet to study physiology?' O’Malley, Dervla; Barry, Denis S.; Rae, Mark G. (2019) 'How much do preclinical medical students utilize the internet to study physiology?'. Advances in Physiology Education, 43 (3):383-391 [DOI] [Full Text] [Details] | |
| (2019) | 'Active peer-mentored learning can improve student understanding of physiological concepts in an undergraduate journal club' Drumm, Bernard T.; Rae, Mark G.; Ward, Sean M. (2019) 'Active peer-mentored learning can improve student understanding of physiological concepts in an undergraduate journal club'. Advances in Physiology Education, 43 (3):359-364 [DOI] [Full Text] [Details] | |
| (2018) | 'No evidence in support of a prodromal respiratory control signature in the TgF344-AD rat model of Alzheimer's disease' Lucking EF;Murphy KH;Burns DP;Jaisimha AV;Barry-Murphy KJ;Dhaliwal P;Boland B;Rae MG;O'Halloran KD; (2018) 'No evidence in support of a prodromal respiratory control signature in the TgF344-AD rat model of Alzheimer's disease'. Respiratory Physiology & Neurobiology, [DOI] [Details] | |
| (2018) | 'The Effect of Superoxide Dismutase Enzyme Inhibition on Renal Microcirculation of Spontaneously Hypertensive-Stroke Prone and Wistar Rats' Ahmeda, AF;Rae, MG;Anweigi, LM;Al Otaibi, MF;Al-Masri, AA;Johns, EJ (2018) 'The Effect of Superoxide Dismutase Enzyme Inhibition on Renal Microcirculation of Spontaneously Hypertensive-Stroke Prone and Wistar Rats'. Physiological Research, 67 :535-541 [Details] | |
| (2017) | 'Using an online student response system, Socrative, to facilitate active learning of Physiology by first year graduate entry to medicine students: a feasibility study' Rae, M.G and O'Malley, D. (2017) 'Using an online student response system, Socrative, to facilitate active learning of Physiology by first year graduate entry to medicine students: a feasibility study'. MedEdPublish, 6 (1) [DOI] [Full Text] [Details] | |
| (2017) | 'Student attitudes towards semi-flipped classroom teaching in a pre-clinical graduate entry to medicine Physiology module' Rae, M.G and O'Malley, D. (2017) 'Student attitudes towards semi-flipped classroom teaching in a pre-clinical graduate entry to medicine Physiology module'. MedEdPublish, [Full Text] [Details] | |
| (2017) | 'An Efficient and Cost-Effective Method of Generating Post-Natal (P2-14) Mouse Primary Hippocampal Neuronal Cultures' Kaar, Aidan; Morley, Shane J.; Rae, Mark G. (2017) 'An Efficient and Cost-Effective Method of Generating Post-Natal (P2-14) Mouse Primary Hippocampal Neuronal Cultures'. Journal of Neuroscience Methods, 286 :69-77 [DOI] [Full Text] [Details] | |
| (2017) | 'The impact of vodcast utilisation upon student learning in the Physiology component of a first year graduate to entry medicine module' Rae, M.G. & McCarthy, M. (2017) 'The impact of vodcast utilisation upon student learning in the Physiology component of a first year graduate to entry medicine module'. Journal of The Scholarship of Teaching and Learning, 17 (2):1-23 [DOI] [Full Text] [Details] | |
| (2016) | 'Leptin modifies the prosecretory and prokinetic effects of the inflammatory cytokine interleukin-6 on colonic function in Sprague-Dawley rats' Buckley, MM;O'Brien, R;Devlin, M;Creed, AA;Rae, MG;Hyland, NP;Quigley, EMM;McKernan, DP;O'Malley, D (2016) 'Leptin modifies the prosecretory and prokinetic effects of the inflammatory cytokine interleukin-6 on colonic function in Sprague-Dawley rats'. Experimental Physiology, 101 :1477-1491 [DOI] [Details] | |
| (2016) | 'Do prerecorded lecture VODcasts affect lecture attendance of first-year pre-clinical Graduate Entry to Medicine students?' Rae, M.G. & O'Malley, D. (2016) 'Do prerecorded lecture VODcasts affect lecture attendance of first-year pre-clinical Graduate Entry to Medicine students?'. Medical Teacher, [DOI] [Full Text] [Details] | |
| (2016) | 'Leptin modifies the prosecretory and prokinetic effects of the inflammatory cytokine interleukin-6 on colonic function in Sprague–Dawley rats' Buckley, Maria M.; O'Brien, Rebecca; Devlin, Michelle; Creed, Aisling A.; Rae, Mark G.; Hyland, Niall P.; Quigley, Eamonn M. M.; McKernan, Declan P.; O'Malley, Dervla (2016) 'Leptin modifies the prosecretory and prokinetic effects of the inflammatory cytokine interleukin-6 on colonic function in Sprague–Dawley rats'. Experimental Physiology, 101 (12):1477-1491 [DOI] [Full Text] [Details] | |
| (2016) | 'Cognitive dysfunction in Duchenne Muscular Dystrophy: a possible role for neuromodulatory immune molecules' Rae MG;O'Malley D; (2016) 'Cognitive dysfunction in Duchenne Muscular Dystrophy: a possible role for neuromodulatory immune molecules'. Journal of neurophysiology, [DOI] [Full Text] [Details] | |
| (2016) | 'Effect of tempol and tempol plus catalase on intra-renal haemodynamics in Spontaneously Hypertensive Stroke-Prone (SHSP) and Wistar rats' Ahmeda, A.F. Rae, M.G., Al Otaibi, M.F., Anweigi, L.M. & Johns, E.J. (2016) 'Effect of tempol and tempol plus catalase on intra-renal haemodynamics in Spontaneously Hypertensive Stroke-Prone (SHSP) and Wistar rats'. Journal Of Physiology And Biochemistry, [DOI] [Full Text] [Details] | |
| (2015) | 'Metabotropic glutamate receptor-mediated cyclic ADP ribose signalling' Kaar, A,Rae, MG (2015) 'Metabotropic glutamate receptor-mediated cyclic ADP ribose signalling'. Biochemical Society Transactions, 43 :405-409 [DOI] [Details] | |
| (2014) | 'Modulation of enteric neurons by interleukin-6 and corticotropin-releasing factor contributes to visceral hypersensitivity and altered colonic motility in a rat model of irritable bowel syndrome' Buckley, MM,O'Halloran, KD,Rae, MG,Dinan, TG,O'Malley, D (2014) 'Modulation of enteric neurons by interleukin-6 and corticotropin-releasing factor contributes to visceral hypersensitivity and altered colonic motility in a rat model of irritable bowel syndrome'. Journal of Physiology-London, 592 :5235-5250 [DOI] [Details] | |
| (2013) | 'Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats' Ahmeda, AF,Rae, MG,Johns, EJ (2013) 'Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats'. Acta Physiologica, 209 :156-166 [DOI] [Details] | |
| (2012) | 'Putative TRP channel antagonists, SKF 96365, flufenamic acid and 2-APB, are non-competitive antagonists at recombinant human alpha 1 beta 2 gamma 2 GABA(A) receptors' Rae, MG,Hilton, J,Sharkey, J (2012) 'Putative TRP channel antagonists, SKF 96365, flufenamic acid and 2-APB, are non-competitive antagonists at recombinant human alpha 1 beta 2 gamma 2 GABA(A) receptors'. Neurochemistry International, 60 :543-554 [DOI] [Details] | |
| (2011) | 'A question of balance - Positive versus negative allosteric modulation of GABA(A) receptor subtypes as a driver of analgesic efficacy in rat models of inflammatory and neuropathic pain' Munro, G;Erichsen, HK;Rae, MG;Mirza, NR (2011) 'A question of balance - Positive versus negative allosteric modulation of GABA(A) receptor subtypes as a driver of analgesic efficacy in rat models of inflammatory and neuropathic pain'. Neuropharmacology, 61 :121-132 [DOI] [Details] | |
| (2011) | 'Effect of clazosentan, a selective endothelin A receptor antagonist, and tezosentan, a dual endothelin A/B antagonist, on pulsatile shear stress induced constriction of the iliac in the anaesthetized pig' Ruane-O'Hora, T;Rae, MG;Markos, F (2011) 'Effect of clazosentan, a selective endothelin A receptor antagonist, and tezosentan, a dual endothelin A/B antagonist, on pulsatile shear stress induced constriction of the iliac in the anaesthetized pig'. Clinical and experimental pharmacology & physiology, 38 :515-520 [DOI] [Details] | |
| (2004) | 'Both mGluR1 and mGluR5 mediate Ca2+ release and inward currents in hippocampal CA1 pyramidal neurons' Rae MG, Irving AJ; (2004) 'Both mGluR1 and mGluR5 mediate Ca2+ release and inward currents in hippocampal CA1 pyramidal neurons'. Neuropharmacology, 46 (8):1057-1069 [DOI] [Details] | |
| (2002) | 'Leptin inhibits rat hippocampal neurons via activation of large conductance. Nat Neurosci' Shanley L.J., Irving, A.J., Rae, M.G., - Ashford, M.L.J.,Harvey, J. (2002) 'Leptin inhibits rat hippocampal neurons via activation of large conductance. Nat Neurosci'. Nature Neuroscience, 5 (4):299-300 [Details] | |
| (2002) | 'Cannabinoids on the brain. ScientificWorldJournal' Irving A.J., Rae, M.G.Rae, Coutts, A. A.; (2002) 'Cannabinoids on the brain. ScientificWorldJournal'. The Scientific World Journal, 2 :632-48 [Details] | |
| (2000) | 'Functional expression of cell surface cannabinoid CB(1) receptors on presynaptic inhibitory terminals in cultured rat hippocampal neurons' Irving AJ, Coutts AA, Harvey J, Rae MG, Mackie K, Bewick GS, Pertwee RG; (2000) 'Functional expression of cell surface cannabinoid CB(1) receptors on presynaptic inhibitory terminals in cultured rat hippocampal neurons'. Neuroscience, 98 (2):253-262 [Details] | |
| (2000) | 'Role of Ca2+ stores in metabotropic L-glutamate receptor-mediated supralinear. J Neurosci' Rae M.G., Martin, D. J., Collingridge, G. L., Irving, A. J. (2000) 'Role of Ca2+ stores in metabotropic L-glutamate receptor-mediated supralinear. J Neurosci'. Journal of Neuroscience, 20 (23):8628-36 [Details] | |
| (1998) | 'Basal activation of ATP-sensitive potassium channels in murine colonic smooth. Biophys J' Koh, S.D.- Bradley, K. K., Rae, M. G., Keef, K. D., Horowitz, B., Sanders, K. M. (1998) 'Basal activation of ATP-sensitive potassium channels in murine colonic smooth. Biophys J'. Biophysical Journal, 75 (4):1793-800 [Details] | |
| (1998) | 'Control of motility patterns in the human colonic circular muscle layer by. J Physiol' Rae M.G., Fleming, N., McGregor, D. B.,Sanders K.M., Keef, K. D. (1998) 'Control of motility patterns in the human colonic circular muscle layer by. J Physiol'. Journal of Physiology-London, 510 ( Pt 1) :309-20 [Details] | |
| (1998) | 'Modulation of cholinergic neuromuscular transmission by nitric oxide in canine. Am J Physiol' Rae M.G., Khoyi, M. A., Keef, K. D. (1998) 'Modulation of cholinergic neuromuscular transmission by nitric oxide in canine. Am J Physiol'. American Journal of Physiology - Gastrointestinal and Liver Physiology, 275 (6 Pt 1) [Details] | |
| (1998) | 'Pharmacological properties of P2X3-receptors present in neurones of the rat dorsal root ganglia' Rae MG;Rowan EG;Kennedy C; (1998) 'Pharmacological properties of P2X3-receptors present in neurones of the rat dorsal root ganglia'. British Journal of Pharmacology, 124 (1) [DOI] [Details] | |
| (1996) | 'Characterization of a P2X-purinoceptor in cultured neurones of the rat dorsal root ganglia' Robertson SJ;Rae MG;Rowan EG;Kennedy C; (1996) 'Characterization of a P2X-purinoceptor in cultured neurones of the rat dorsal root ganglia'. British Journal of Pharmacology, 118 (4) [DOI] [Details] | |
| (1996) | 'Neuronal mediators of inhibitory junction potentials and relaxation in the. J Physiol' Rae M.G., Muir, T. C. (1996) 'Neuronal mediators of inhibitory junction potentials and relaxation in the. J Physiol'. Journal of Physiology-London, 493 ( Pt 2) :517-27 [Details] | |
| (1992) | 'Regulation of prostaglandin E2 binding to a murine macrophage cell line, P388D1. Biochim Biophys Acta' Rae M.G., Rotondo, D., Dutta-Roy, A. K.; (1992) 'Regulation of prostaglandin E2 binding to a murine macrophage cell line, P388D1. Biochim Biophys Acta'. Biochem Biophys Acta, 1135 (3):343-8 [Details] | |
| (1992) | 'Interleukin-1 inhibits PGE2 binding to macrophage-like P388D1 cells by a cyclic. Biochim Biophys Acta' Rae M.G., Rotondo D., Milton, A. S., Dutta-Roy, A. K. (1992) 'Interleukin-1 inhibits PGE2 binding to macrophage-like P388D1 cells by a cyclic. Biochim Biophys Acta'. Biochem Biophys Acta, 1138 (1):75-9 [Details] | |
| (1991) | 'Stimulation of prostaglandin E2 binding to a murine macrophage cell line (P388D1) by insulin' Dutta-Roy AK, Rae MG, Milton AS, Rotondo D; (1991) 'Stimulation of prostaglandin E2 binding to a murine macrophage cell line (P388D1) by insulin'. Biochemical Society Transactions, 19 (4) [Details] |
Conference Publications
| Year | Publication | |
|---|---|---|
| (2019) | RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY Lucking, EF;Murphy, KH;Burns, DP;Jaisimha, AV;Barry-Murphy, KJ;Dhaliwal, P;Boland, B;Rae, MG;O'Halloran, KD (2019) No evidence in support of a prodromal respiratory control signature in the TgF344-AD rat model of Alzheimer's disease RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY , pp.55-67 [DOI] [Details] | |
| (2014) | Biochemical Society KAAR, A. & RAE, M.G. (2014) Investigating the role of the cyclic ADP ribose/RyR pathway in Group I mGluR-mediated signalling in cultured rat hippocampal neurones Biochemical Society [Details] | |
| (2014) | Royal Academy of Medicine, Ireland KAAR, A., O’DONOGHUE, K. & RAE, M.G (2014) Investigating the role of cyclic ADP ribose in group I metabotropic glutamate receptor - mediated calcium signalling in rat hippocampal neurones Royal Academy of Medicine, Ireland [Details] | |
| (2013) | Neuroscience Ireland KAAR, A., LEAHY, J., O’DONOGHUE, K. & RAE, M.G (2013) Evidence for the involvement of ryanodine receptors in Ca2+ release by group I metabotropic glutamate receptors in cultured rat hippocampal neurons Neuroscience Ireland [Details] | |
| (2013) | Royal Academy of Medicine, Ireland LEAHY, J., KAAR, A. & RAE, M.G (2013) Evidence for the involvement of cyclic ADP ribose-dependent endoplasmic reticulum Ca2+ release by group I metabotropic glutamate receptors in cultured rat hippocampal neurons Royal Academy of Medicine, Ireland [Details] | |
| (2012) | Alzheimers Dement MORLEY, S.J., KAAR, A. & RAE, M.G. (2012) Evoked hippocampal supralinear intracellular calcium responses are absent in 3xTg Alzheimer's mice Alzheimers Dement [Details] | |
| (2010) | Neuroscience Ireland 2010 Rae, MG; Thompson, J; Sharkey, J; (2010) The putative TRP channel antagonists, SKF96365, flufenamic acid and 2-APB are non-competitive antagonists at recombinant alpha1, beta2, gamma2 GABAA receptors Neuroscience Ireland 2010 [Details] | |
| (2011) | Royal Academy of Medicine, Ireland MORLEY, S.J., KEOGH, M.K.& RAE, M.G (2011) Evidence of altered intracellular calcium handling in week old cultured hippocampal neurones of a murine model of Alzheimer’s disease (3xTg AD) Royal Academy of Medicine, Ireland [Details] | |
| (2003) | J. Physiol RAE, M.G. & IRVING, A.J.; (2003) Pharmacology of the group I metabotropic receptor-mediated responses in rat CA1 hippocampal neurones J. Physiol [Details] | |
| (2003) | J. Physiol WHITE, A.M., RAE, M.G., IRVING, A.J. & PLATT, B.; (2003) Group I mGluR-induced calcium signalling in the rat superficial superior colliculus J. Physiol [Details] | |
| (2003) | Soc. Neuroscience Abs RAE, M.G & IRVING, A.J. ; (2003) Metabotropic glutamate receptors (mGluRs) and dynamic endoplasmic reticulum (ER) loading in CA1 pyramidal neurons Soc. Neuroscience Abs [Details] | |
| (2001) | Soc. Neuroscience Abs RAE, M.G. & IRVING, A.J.; (2001) Relationship between group I metabotropic glutamate receptor-activated inward currents and supralinear calcium signalling in hippocampal neurons Soc. Neuroscience Abs [Details] | |
| (2001) | Int. Cannabinoid Res. Soc IRVING, A.J., CALDWELL, D., RAE, M.G., BEWICK, G & COUTTS A.A.; (2001) Functional expression of cell surface CB1 receptors at synaptic terminals in cultured rat cerebellar granule cells Int. Cannabinoid Res. Soc [Details] | |
| (2000) | J. Physiol RAE, M.G. & IRVING, A.J.; (2000) A novel coincidence detection mechanism involving mGlu receptors in the rat hippocampus J. Physiol [Details] | |
| (1999) | J. Physiol RAE, M.G. & IRVING, A.J.; (1999) Effects of cyclopiazonic acid on intracellular Ca2+ levels in cultured hippocampal neurones J. Physiol [Details] | |
| (1997) | Gastroenterology RAE, M.G., KHOYI, M. & KEEF, K.D; (1997) Interaction between cholinergic and nitrergic transmission in the canine colonic circular muscle layer Gastroenterology [Details] | |
| (1996) | Drug Development Research RAE, M.G., ROWAN, E.G. & KENNEDY, C.; (1996) Further characterisation of P2X-purinoceptors in cultured neurones of the rat dorsal root ganglia Drug Development Research [Details] | |
| (1996) | Br. J. Pharmacol RAE, M.G., ROWAN, E.G. & KENNEDY, C. ; (1996) Characterisation of P2X-purinoceptors in neurones of the neonatal rat dorsal root ganglia Br. J. Pharmacol [Details] | |
| (1995) | Br. J. Pharmacol RAE, M.G. & MUIR, T.C. ; (1995) Suramin reveals two distinct neuronally-mediated IJP components in the guinea-pig internal anal sphincter (gpIAS) Br. J. Pharmacol [Details] | |
| (1995) | Neurogastroenterology and Motility RAE, M.G., QUINN, P. & MUIR, T.C. ; (1995) Alterations in both cAMP and cGMP levels of the guinea-pig internal anal sphincter (gpIAS) in response to neuronal stimulation and drugs Neurogastroenterology and Motility [Details] | |
| (1994) | Br. J. Pharmacol RAE, M.G. & MUIR, T.C.; (1994) Both ATP and Nitric Oxide (NO) may be involved in the neuronally-mediated relaxation of the guinea-pig internal anal sphincter (gpIAS) Br. J. Pharmacol [Details] | |
| (1993) | J. Gastrointestinal Motility RAE, M.G. & MUIR, T.C.; (1993) Neuronally-mediated inhibition in the guinea-pig internal anal sphincter (gpIAS) ¿ indirect nitric oxide involvement J. Gastrointestinal Motility [Details] | |
| (1992) | Congress of the International Union of Physiological Sciences XXXII SMITH, A.D., RAE, M.G. & MUIR, T.C.; (1992) Excitation and inhibition by nitric oxide (NO) in smooth muscle Congress of the International Union of Physiological Sciences XXXII [Details] | |
| (1992) | Br. J. Pharmacol RAE, M.G. & MUIR, T.C. ; (1992) Nitrovasodilators potentiate and inhibit spontaneous electrical and mechanical activity in the guinea-pig internal anal sphincter (gpIAS) Br. J. Pharmacol [Details] |
Abstract
| Year | Publication | |
|---|---|---|
| (2017) | Respiratory Control in a Rat Model of Alzheimer's Disease (TgF344-AD). Murphy, KH;Burns, DP;Barry-Murphy, K;Rae, MG;O'Halloran, KD;Lucking, EF (2017) Respiratory Control in a Rat Model of Alzheimer's Disease (TgF344-AD). BETHESDA: Abstract [Details] | |
| (2017) | Early calcium signalling remodelling in Alzheimer's disease - evidence from murine cultured hippocampal neurons. Kaar, A;Rae, MG (2017) Early calcium signalling remodelling in Alzheimer's disease - evidence from murine cultured hippocampal neurons. HOBOKEN: Abstract [Details] | |
| (2016) | Evidence for the Involvement of Cyclic ADP Ribose-Dependent Endoplasmic Reticulum Ca2+ Release by Group I Metabotropic Glutamate Receptors in Cultured Rat Hippocampal Neurons. Leahy, J;Kaar, A;Rae, MG (2016) Evidence for the Involvement of Cyclic ADP Ribose-Dependent Endoplasmic Reticulum Ca2+ Release by Group I Metabotropic Glutamate Receptors in Cultured Rat Hippocampal Neurons. LONDON: Abstract [Details] | |
| (2016) | Investigating the Role of Cyclic ADP Ribose in Group I Metabotropic Glutamate Receptor-Mediated Calcium Signalling in Rat Hippocampal Neurones. Kaar, A;O'Donoghue, K;Rae, MG (2016) Investigating the Role of Cyclic ADP Ribose in Group I Metabotropic Glutamate Receptor-Mediated Calcium Signalling in Rat Hippocampal Neurones. LONDON: Abstract [Details] | |
| (2016) | Investigating the Involvement of the Cyclic ADP Ribose Signalling Pathway in Group I Metabotropic Glutamate Receptor-Mediated Signalling, in Cultured Murine Hippocampal Neurones. Kaar, A;Rae, MG (2016) Investigating the Involvement of the Cyclic ADP Ribose Signalling Pathway in Group I Metabotropic Glutamate Receptor-Mediated Signalling, in Cultured Murine Hippocampal Neurones. LONDON: Abstract [Details] | |
| (2016) | Dysregulation of calcium homeostasis and signalling in cultured hippocampal neurones from young rodent models of Alzheimer's disease (3xTg-AD mouse & TgF344-AD rat). Kaar, A;Rae, MG (2016) Dysregulation of calcium homeostasis and signalling in cultured hippocampal neurones from young rodent models of Alzheimer's disease (3xTg-AD mouse & TgF344-AD rat). HOBOKEN: Abstract [Details] | |
| (2011) | EVIDENCE OF ALTERED INTRACELLULAR CALCIUM HANDLING IN WEEK OLD CULTURED HIPPOCAMPAL NEURONES OF A MURINE MODEL OF ALZHEIMER'S DISEASE (3XTg AD). Morley, SJ;Keogh, MK;Rae, MG (2011) EVIDENCE OF ALTERED INTRACELLULAR CALCIUM HANDLING IN WEEK OLD CULTURED HIPPOCAMPAL NEURONES OF A MURINE MODEL OF ALZHEIMER'S DISEASE (3XTg AD). LONDON: Abstract [Details] | |
| (2011) | THE PUTATIVE TRP CHANNEL ANTAGONISTS, SKF96365, FLUFENAMIC ACID AND 2-APB ARE NON-COMPETITIVE ANTAGONISTS AT RECOMBINANT alpha(1)beta(1)gamma(2) GABA(A) RECEPTORS. Rae, MG;Thompson, J;Sharkey, J (2011) THE PUTATIVE TRP CHANNEL ANTAGONISTS, SKF96365, FLUFENAMIC ACID AND 2-APB ARE NON-COMPETITIVE ANTAGONISTS AT RECOMBINANT alpha(1)beta(1)gamma(2) GABA(A) RECEPTORS. LONDON: Abstract [Details] |
Professional Activities
Honours and Awards
| Year | Title | Awarding Body | |
|---|---|---|---|
| 2018 | Supervisee (Michael Vaughan) winner of The Physiological Society Rob Clarke Undergraduate Award | The Physiological Society | |
| 2018 | Supervisee (Kimberley Stephenson) winner of Young Investigator best poster prize at Europhysiology 2018 | The Physiological Society | |
| 2007 | Awarded place on Molecular Techniques Workshop | The Physiological Society | |
| 2012 | Supervisee (Shane Morley) awarded Physiological Society Student Travel Bursary | Physiological Society | |
| 2014 | Supervisee (Aidan Kaar) awarded UCC, College of Medicine and Health Doctoral Student Travel Bursary | UCC | |
| 2014 | Supervisee (Aidan Kaar) awarded Biochemical Society Student Travel Bursary | The Biochemical Society | |
| 2015 | Supervisee (Aidan Kaar) awarded Federation of European Neurosciences (FENS) - Society for Neuroscience (SfN) bursary. | Federation of European Neurosciences and Society for Neuroscience | |
| 2016 | Biochemical Society Travel Award | The Biochemical Society | |
| 2016 | Physiological Society Travel Award | The Physiological Society | |
| 2016 | Outstanding lecturer in Years 1 & 2 of the Graduate Entry Medicine Programme | UCC School of Medicine |
Professional Associations
| Association | Function | From / To | |
|---|---|---|---|
| Cork Neuroscience Centre | member | 06-OCT-07 / | |
| The European Calcium Society | member | 01-JUL-16 / | |
| Royal Academy of Medicine Ireland | member | 01-OCT-15 / | |
| Neuroscience Ireland | member | 01-JUL-08 / | |
| Biochemical Society | member | 01-JUL-14 / | |
| The Physiological Society | member and UCC Physiological Society Representative | 07-OCT-00 / | |
| Alzheimer's Association | member | 02-JAN-12 / |
Committees
| Committee | Function | From / To | |
|---|---|---|---|
| Department of Physiology Graduate Studies Committee | member | 2007 / | |
| Department of Physiology Research Committee | 2007 / | ||
| Graduate Entry Medicine Steering Group. | 2007 / 2008 | ||
| Graduate Entry Medicine Teaching and Oversight Committee | Module coordinator - GM1001 | 2008 / | |
| School of Medicine Student Affairs Committee | 2009 / | ||
| Graduate Entry Medicine Curriculum Review Committee. | GM1001 module coordinator | 2010 / |
Journal Activities
| Journal | Role | To / From | |
|---|---|---|---|
| Brain Research Bulletin | Referee | 01-AUG-12 - | |
| Pharmacologia | Editor | 01-NOV-13 - | |
| International Journal Of Neurology Research | Member of Editorial Board | 02-NOV-14 - | |
| Alzheimers & Dementia | Referee | 11-JAN-17 - | |
| Journal Of The Scholarship Of Teaching And Learning | Referee | - | |
| British Journal Of Pharmacology | Referee | - | |
| Journal Of Physiology | Referee | - | |
| Journal Of Toxicology | Referee | - | |
| Journal Of Endocrinology | Referee | - |
Employment
| Employer | Position | From / To | |
|---|---|---|---|
| University College Cork | College Lecturer in Physiology | 17-JUL-08 / | |
| University College Cork | Contract Lecturer | 01-JUL-07 / 01-OCT-08 | |
| Astellas CNS in Edinburgh | Senior Electrophysiologist | 01-JAN-06 / 01-FEB-07 | |
| Ionix Pharmaceuticals Ltd. | Research Electrophysiologist | 01-NOV-04 / 01-SEP-05 |
Outreach Activities
| Description | |
|---|---|
Organiser of, and participant in, ‘Physiology Friday’, a fun day designed to promote awareness of the discipline of Physiology across the larger university community through physiological challenges, quizzes, a Physiology-themed bake sale (Biobakes) and talent shows (UCC, November 2015 & 2016). (https://www.ucc.ie/en/physiology/news/fullstory-717577-en.html) Co-organiser of Physiology Department’s 2016 contribution to Cork Science Festival - ‘Can you beat Usain Bolt out of the blocks?’ http://www.corksciencefestival.ie/celebrate-science-open-day-13th-november/ |
Education
| Year | Institution | Qualification | Subject | |
|---|---|---|---|---|
| 1991 | Aberdeen University | BSc | Pharmacology | |
| 1995 | Glasgow University | PhD | Physiology/Pharmacology | |
| 2013 | University College Cork | Certificate in Teaching and Learning in Higher Edu | Teaching and Learning in Higher Education | |
| 2015 | University College Cork | Postgraduate Diploma in Teaching and Learning in Higher Education | Teaching and Learning in Higher Education | |
| 2016 | University College Cork | Masters in Teaching and Learning in Higher Education | Teaching and Learning in Higher Education |
Other Activities
| Description | |
|---|---|
Invited external examiner (2016): PhD thesis - Stephen Fedigan, Dundalk Institute of Technology. | |
Appointed as a Teaching Fellow for the Centre for the Integration of Research, Teaching and Learning (CIRTL) here at UCC in 2017. | |
Invited internal examiner (2014): MSc thesis - Nurullah Morshed, Department of Physiology, UCC. | |
Invited internal examiner (2013): MSc thesis - Nura Benghezzi, Department of Physiology, UCC. | |
Departmental representative for The Physiological Society https://www.physoc.org/ | |
Grant reviewer for Alzheimer's Assocaition, U.S.A. | |
Member of Asian Council of Science Editors (http://theacse.com/) | |
Developed and manage Physiology department’s online question database server with which one can produce and run on-line Physiology examinations in all required formats, e.g. True/False, MCQ, SBA, EMQ. | |
Invited to join COST action group ‘Personalized Nutrition in aging society: redox control of major age-related diseases’ (2016). |
Teaching Activities
Teaching Interests
Module coordinator for:
GM1001, PL4011
Physiology departmental coordinator for:
GM1001, GM1002, GM1003 & GM2001
Teach on:
GM1001, GM1002, GM1003, GM2001, PL3025, PL4011 & PL4020
Undergraduate Teaching
I have teaching experience across a variety of years and undergraduate modules including 2nd, 3rd and 4th year science and 1st and 2nd and final year direct entry and graduate entry medicine (GEM). I currently deliver undergraduate modules on the 1st and 2nd year GEM course, and 3rd and 4th year B.Sc. Physiology (7 modules in total) using a variety of teaching formats such as formal lectures, tutorials, small group learning (SGL), student projects and laboratory practicals
Details of my current teaching on individual modules are listed below.Graduate & Direct Entry to Medicine Teaching
GM 1001 (Module Coordinator), Physiology coordinator for GM 1002,GM 1003 & GM 2001
The bulk of my teaching is devoted to the Graduate Entry to Medicine (GEM) course. I teach 50% of the Physiology contribution on all three of the 1st year basic science modules as well as making a significant contribution to the 2nd year GM2001 module. I am module coordinator for the 20 credit GM1001 module with responsibility for coordinating input from the various contributory departments, timetabling, exam preparation and marking and am Physiology coordinator for the 1st year GEM course.
I also act as a facilitator for the small group learning (SGL) tutorial sessions.
3rd Year B.Sc. Physiology Teaching
PL3025 Literature Review, Experimental Design and Data Analysis
In this 5 credit module students either write a report on an original peer-reviewed research article, a literature review of a particular Physiological topic or a report on a proposed experimental protocol designed to investigate a particular Physiological question. This is an important introduction to the scientific literature and paves the way for research projects in year 4.
4th Year B.Sc. Physiology Teaching
PL4011 (Learning & Memory)
In this5 credit module I lecture on my own research field of learning and memory, which is directly informed by my work into the pathogenesis of Alzheimer’s disease (AD) and Down Syndrome (DS).
PL4020 (Laboratory Research and/or Literature Review Project)
The PL4020 module (20 credits) is a 12-16 week research-led, major component of the final year Physiology honours degree. I currently provide three lab-based projects relevant to, and informed by, my own lab-based research interests.
Recent Postgraduates
| Graduation Year | Student Name | Institution | Degree Type | Thesis Title | |
|---|---|---|---|---|---|
| 2017 | Shane Morley | University College Cork | Ph.D. | Acetylated microtubules are essential for touch sensation in mice | |
| 2014 | Shane Morley | University College Cork | MASTER OF SCIENCE | Study of Intracellular Calcium Dynamics in Hippocampal Neurons from a Murine Model of Alzheimer’s Disease. | |
| 2019 | Anirudh Vinay Jaisimha | University College Cork | Ph.D. | Examining potential causes of neuronal dysfunction in Alzheimer’s disease | |
| 2019 | Aidan Kaar | University College Cork | Ph.D. | nvestigating neuronal calcium homeostasis in murine models of Alzheimer’s disease  | |
| 2015 | William Murphy | University College Cork | MASTER OF SCIENCE | Bio-Impedimetric and Electrophysiological Analysis to Screen Potential Anti-Cancer Compounds in Colon Cancer Treatment |
Current Postgraduate Students
| Student | Degree Type | |
|---|---|---|
| Jaisimha Anirudh Vinay | Doctoral Degree | |
| Vaughan Michael Bernard | Doctoral Degree |
Modules Taught
| Term (ID)) | Title | Link | Subject | |
|---|---|---|---|---|
| 2020 | Learning and Memory | PL4011 | Learning and Memory | |
| 2020 | Fundamentals of Medicine I | GM1001 | Fundamentals of Medicine I |
Research Information
Internal Collaborators
| Name | Institute | Country | |
|---|---|---|---|
| Barry Boland | Department of Pharmacology & Therapeutics, University College Cork | IRELAND | |
| Brian McSharry | Department of Microbiology, University College Cork | IRELAND | |
| Ruslan Dmitriev | Department of Biochemistry, University College Cork | IRELAND | |
| Suzanne Timmons | Department of Gerentology, University College Cork | IRELAND | |
| John Mackrill | Department of Physiology, University College Cork | IRELAND | |
| Ken O'Halloran | Department of Physiology, University College Cork | IRELAND | |
| Dervla O'Malley | Department of Physiology, University College Cork | IRELAND | |
| Harriet Schellekens | Department of Anatomy & Neuroscience, University College Cork | IRELAND |
External Collaborators
| Name | Organisation / Institute | Country | |
|---|---|---|---|
| Geert Bultynck | KU Leuven | BELGIUM | |
| Sean Kennelly | Tallaght Hospital, Dublin | IRELAND | |
| Katsuhiko Mikoshiba | RIKEN Brain Science Institute | JAPAN | |
| Eric Moore | Tyndall National Institute, Cork | IRELAND |